Cancer Metabolism

1 Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction

Daniel R. Crooks, Teresa W-M. Fan, and W. Marston Linehan

2 Imaging Cancer Metabolism with Positron Emission Tomography (PET)

Timothy H. Witney and David Y. Lewis

3 Radioluminescence microscopy - A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells

Debanti Sengupta and Guillem Pratx

4 Use of 13C315N1-Serine or 13C515N1-Methionine for Studying Methylation dynamics in Cancer Cell Metabolism and Epigenetics

Tong Zhang, Christiaan F. Labuschagne, Karen H. Vousden, and Oliver D. K. Maddocks

5 Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM)

Sarah Creed and Matthew McKenzie

6 Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-induced Signalling in Cancer Cells

David Kung-Chun Chiu, Misty Shuo Zhang, Aki Pui-Wah Tse, and Carmen Chak-Lui Wong

7 Determination of Polarization of Resident Macrophages and their Effect on the Tumor Microenvironment

Ioannis S. Pateras and Tomer Cooks

8 Quantitation of Macropinocytosis in Cancer Cells

Koen M.O. Galenkamp, Basheer Alas, and Cosimo Commisso

9 Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate Metabolically Driven Acetylation

Simone Sidoli, Sophie Trefely, Benjamin A. Garcia, and Alessandro Carrer

10 Methods to Measure Autophagy in Cancer Metabolism

Natalia Von Muhlinen

11 Lipidomic Analysis of Cancer Cell and Tumor Tissue

Sk Ramiz Islam and Soumen Kanti Manna

12 Mass Spectrometry-based Profiling of Metabolites in Human Biofluids

Tanushree Chakraborty and Soumen Kanti Manna 13 Gas Chromatography-Mass Spectrometry