Genetic Library Construction and Screening
Advanced Techniques and Applications
(Sprache: Englisch)
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Inhaltsverzeichnis zu „Genetic Library Construction and Screening “
PCR and cDNA Cloning Techniques.- 1 Strategies for cDNA Cling and Mapping RNA Transcripts.- 2 Cloning PCR Products.- 3 Site Directed Deletion, Insertion, and Substitution Using PCR.- 4 Long RT-PCR Cloning - Amplification of Full-Length Enterovirus Genomes.- 5 Construction of cDNA Libraries from Small Quantities of Total RNA Using Template Switching Catalyzed by M-MLV Reverse Transcriptase.- Subtractive and Differential Display Techniques.- 6 Subtractive Hybridization and cDNA Cloning.- 7 Differential Display to Identify Steroid-Induced Genes in Endocrine Cells.- Specialized Cloning and Library Screening Strategies.- 8 Two Hyrid cDNA Cloning.- 9 Yeast One and Two Hyrid cDNA Cloning.- 10 High-Throughput Library Screening by Fluorescent Hybridisations on Gridded Membranes.- 11 Identification of Cell Targeting Ligands Using Random Peptide-Presenting Phage Libraries.
Bibliographische Angaben
- 2012, 2002, XIII, 241 Seiten, Maße: 15,8 x 23,7 cm, Kartoniert (TB), Englisch
- Herausgegeben: R.C. Bird, Bruce F. Smith
- Verlag: Springer, Berlin
- ISBN-10: 364247733X
- ISBN-13: 9783642477331
Sprache:
Englisch
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