Methods Used in Adenosine Research

I Synthesis and Measurement of Adenosine and Adenine Nucleotide Analogs.- 1 Synthesis of Adenosine and Adenine Nucleotide Analogs.- 2 The Measurement of Adenosine and Adenine Nucleotides in Tissues and Body Fluids.- 3 The Demonstration and Measurement of Adenosine Triphosphate Release from Nerves.- II Adenosine Metabolism.- 4 The Study of Adenosine Metabolism in Isolated Cells and Tissues.- 5 Ectonucleotidases: Measurement of Activities and Use of Inhibitors.- 6 Adenosine Deaminase: Measurement of Activity and Use of Inhibitors.- 7 S-Adenosylhomocysteine Hydrolase: Measurement of Activity and Use of Inhibitors.- 8 Purine Nucleoside Phosphorylase: Measurement of Activity and Use of Inhibitors.- III Adenosine Transport.- 9 Measurement and Inhibition of Membrane Transport of Adenosine.- 10 The Use of Ligands in the Study of the Nucleotide-Transport Complex: Nitrobenzylthioinosine.- IV Classification and Identification of Receptors for Adenosine and Adenine Nucleotides.- 11 The Classification of Receptors for Adenosine and Adenine Nucleotides.- 12 The Pharmacological Estimation of Potencies of Agonists and Antagonists in the Classification of Adenosine Receptors.- 13 Use of Radioligands in the Identification, Classification, and Study of Adenosine Receptors.- 14 Use of Photoaffinity Labels as P2-Purinoceptor Antagonists.- 15 Use of Structure-Activity Relationships in the Study of Adenosine Receptors.- 16 Classification of Adenosine Receptors in the Central Nervous System.- 17 Classification of Adenosine Receptors in Peripheral Tissues.- V Physiological Role of Adenosine.- 18 Criteria for the Involvement of Adenosine in the Regulation of Blood Flow.- 19 Methods Used to Study the Involvement of Adenosine in the Regulation of Lipolysis.- 20 Criteria for the Involvement of Adenosine and Adenine Nucleotides in Nonadrenergic, Noncholinergic Transmission.