Osteoporosis

- In vitro models

1. Isolation and Culture of Rodent Osteoprogenitor Cells
Thomas A. Owen* and Lydia C. Pan

2. Osteoclast Culture and Resorption Assays
Elizabeth W. Bradley and Merry Jo Oursler*

3. Assessing New Bone Formation in Neonatal Calvarial Organ Cultures
Khalid S. Mohammad, John M. Chirgwin and Theresa Guise*

4. Detection of Apoptosis of Bone Cells In Vitro
Teresita Bellido*and Lilian I. Plotkin

In vivo rodent models

5. In Vivo Parathyroid Hormone Treatments and mRNA Isolation and Analysis
Xin Li, Ling Qin and Nicola C. Partridge*

6. Assessment of Bone Formation Capacity Using In Vivo Transplantation Assays: Procedure and Tissue Analysis
Badem M. Abdalleh, Nicholas Ditzel and Moustapha Kassem*

7. Ossicle and Vossicle Implant Model Systems
Glenda J. Pettway and Laurie K. McCauley*

8. Utility of the Ovariectomized (OVX) Rat as a Model for Human Osteoporosis In Drug Discovery
Yogendra P. Kharode*, Michael C. Sharp and Peter V. N. Bodine

9. Orchidectomy (ORX) Models of Osteoporosis

Stéphane Blouin, Hélène Libouban, Marie Françoise Moreau and Daniel Chappard*

Methods of gene delivery

10. Gene Delivery by Adenoviruses
Renny Franceschi* and Chunxi Ge

11. Lentiviral Delivery of shRNA Constructs into Osteoblasts
Marc N. Wein, Dallas C. Jones and Laurie H. Glimcher*

12. Gene Delivery by Retroviruses
Valerie Deregowski and Ernesto Canalis*

Molecular and GeneticTechniques

13. Chromatin Immunoprecipitation (ChIP) Assays: Application of ChIP-on-chip for Defining Dynamic Transcriptional Mechanisms in Bone Cells
Margaret van der Deen, Mohammad Q. Hassan, Jitesh Pratap, Nadiya M. Teplyuk, Daniel W. Young, Amjad Javed, Sayyed K. Zaidi, Jane B. Lian, Martin Montecino, Janet L. Stein, Gary S. Stein and Andre J. van Wijnen*

14. Identification of Transcription Factor Target Genes by ChIP Display (CD)
Artem Barski, Steve Pregizer and Baruch Frenkel*

15. Application of Laser Capture Microdissection (LCM)