RNA

The Transcriptional Landscape.- Working with RNA.- Synthesis of RNA by In vitro Transcription.- Efficient Poly(A)+ RNA Selection Using LNA Oligo(T) Capture.- Genome Browsers.- Web-Based Tools for Studying RNA Structure and Function.- Northern Blotting Analysis.- Rapid Amplification of cDNA Ends (RACE).- Fractionation of mRNA Based on the Length of the Poly(A) Tail.- Analysis of Mutations that Influence Pre-mRNA Splicing.- S1 Nuclease Analysis of Alternatively Spliced mRNA.- Promises and Challenges in Developing RNAi as a Research Tool and Therapy.- Inhibition of Gene Function in Mammalian Cells Using Short-Hairpin RNA (shRNA).- Validation of RNAi by Real Time PCR.- Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method.- The Post-Transcriptional Operon.- RIP-Chip Analysis: RNA-Binding Protein Immunoprecipitation-Microarray (Chip) Profiling.- Isolation of RNP Granules.- Electrophoretic Mobility Shift Assay for Characterizing RNA-Protein Interaction.- Polysome Analysis and RNA Purification from Sucrose Gradients.- Prediction of Targets for microRNAs.- Outsourcing of Experimental Work.- ESM1: In-Line Probing Analysis of Riboswitches.- ESM2: Analysis of the RNA Backbone: Structural Analysis of Riboswitches by In-Line Probing and Selective 2'-Hydroxyl Acylation and Primer Extension (SHAPE).