The Gene and its Engineering

Part I. The Gene, Its Replication and Its Expression

Chapter 1. Concept of a Gene (H. K. Das)

Chapter 2. DNA as the Genetic Material (H. K. Das)

Chapter 3. Constituents of Nucleic Acids (H. K. Das)

Chapter 4. Structure of DNA (H. K. Das)

Chapter 5.Characteristics of DNA (H. K. Das)

Chapter 6. Chromatin and Its Structure (H. K. Das)

Chapter 7. Chromatin Remodelling (Rohini Muthuswami)

Chapter 8. Classic Experiments on DNA Replication In Vivo (H. K. Das)

Chapter 9. DNA Polymerases (H. K. Das)

Chapter 10. Mechanics of DNA Replication (Pramod C Rath)
Chapter 11. RNA, Its Transcription and Processing (Shyamal Goswami)

Chapter 12. How Transcription of Genes Is Switched On and Off (Shyamal Goswami)

Chapter 13. Structure and Replication of RNA (Pramod Kumar Yadav)

Chapter 14. Translation of mRNA into Protein ? Classic and Recent Experiments (
Jayanta Kumar Pal and Sangeeta Chatterjee)

Chapter 15. Translation Regulation of Gene Expression and Human Diseases Jayanta Kumar Pal and Sangeeta Chatterjee)

Chapter 16. Non-coding RNA (Beena Pillai)

Part II. Engineering of Gene

Chapter 17. Cloning of Gene ? How It All Began (H. K. Das)

Chapter 18. Reaction of the Scientific Community after Successful Cloning of a Gene (H. K. Das)

Chapter 19. Genetic Engineering Guidelines (H.K.Das)

Chapter 20. Tools Used in Genetic Engineering (H. K. Das)
Chapter 21. General Strategies for Expression of Introduced Genes in a Foreign Host (Shweta Sharma and Indranil Dasgupta)

Chapter 22. Gram-Negative Bacterium Escherichia coli as Host (H. K. Das)

Chapter 23. Gram-Positive Bacterium Bacillus subtilis as Host (H. K. Das)

Chapter 24. Streptomyces as Host (K. Dharmalingam and J. Jeya Maheshwari)

Chapter 25. Yeast Pichia pastoris as Host (Navin Khanna)

Chapter 26. Silkworm Cells as Host (Krishnaveni Mohareer and Syed Ehtesham Hasnain)

Chapter 27. Animal Cells as Host (Vijay Kumar)

Chapter 28. Plants as Host (Arun Kumar

Prof. Hirendra Kumar Das started his academic career with a master?s degree in Chemical Technology from Calcutta University, India. His Ph.D. was in the area of amino acid metabolism in mitochondria and D.Sc. in the area of protein biosynthesis in mitochondria.
He was an Honorary Lecturer in the Department of Biochemistry, Calcutta University from 1955 to 1964. His initiation to Molecular Genetics and Molecular Biology of Escherichia coli was in 1964, when he joined the group of Prof. Avram Goldstein in Stanford University. He continued to work with Escherichia coli even after his return to India in 1967, where he started teaching Molecular Biology.
In 1980 he switched to Azotobacter vinelandii and initiated work on molecular genetics of nitrogen fixation. He established a Genetic Engineering Unit related to nitrogen fixation research at the Jawaharlal Nehru University, New Delhi, India in 1983 and began teaching Genetic Engineering.
In 1989 he was a Visiting Professor in the Department of Microbiology in the University of Virginia at Charlottesville. During 1990-1991 he served as the Chairman of the Centre for Biotechnology in Jawaharlal Nehru University, New Delhi, India.
Prof. Das?s teaching career spans 40 years, of which 31 years was spent in teaching Molecular Biology and Genetic Engineering. He has served in the editorial Board of the Indian Journal of Biochemistry and Biophysics. Prof. Das is a Fellow of the Indian National Science Academy.