Biophysical Chemistry of Proteins

Part One: Analytical techniques 1.Microscopy 2.Single molecule techniques 3.Preparation of cells and tissues for microscopy 4.Principles of optical spectroscopy 5.Photometry 6.Fluorimetry 7.Chemiluminescence 8.Electrophoresis 9.Immunological methods 10.Isotope techniques Part Two: Purification of proteins 11.Homogenisation and fractionisation of cells and tissues 12.Isolation of organelles 13.Precipitation methods 14.Chromatography 15.Membrane proteins 16.Determination of protein concentration 17.Cell culture Part Three: Protein modification and inactivation 18.General technical remarks 19.Amine-reactive reagents 20.Thiol- and disulphide reactive reagents 21.Reagents for other groups 22.Cross-linkers 23.Detection methods 24.Spontaneous reactions in proteins Part Four: Protein size and shape 25.Centrifugation 26.Osmotic pressure 27.Diffusion 28.Viscosity 29.Non-resonant interactions with electromagnetic waves Part Five: Protein structure 30.Protein sequencing 31.Synthesis of peptides 32.Protein secondary structure 33.Structure of protein-ligand complexes 34.3D-structures 35.Folding and unfolding of proteins Part Six: Enzyme kinetics 36.Steady-state kinetics 37.Leaving the steady state: Analysis of progress curves 38.Reaction velocities 39.Isotope effects 40.Isotope exchange Part Seven: Protein-ligand interactions 41.General conditions for interpretable results 42.Binding equations 43.Methods to measure binding equilibria 44.Temperature effects on binding equilibrium and reaction rate Part Eight: Industrial enzymology 45.Industrial enzyme use 46.Immobilised enzymes Part Nine: Special statistics 47.Quality control 48.Testing whether or not a model fits the data Part Ten: Appendix 49.List of symbols 50.Greek alphabet 51.Properties of electrophoretic buffers 52.Bond properties 53.Acronyms

Dr. Buxbaum's research interests are enzymology and protein structure/function relationship. In addition, he has been teaching science and medical students in several countries. He is currently working as associate professor of biochemistry at Ross University School of Medicine.