Cell-Based Assays for High-Throughput Screening
Methods and Protocols
(Sprache: Englisch)
As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an...
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As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology(TM) series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.
Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.
Klappentext zu „Cell-Based Assays for High-Throughput Screening “
As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology(TM) series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.
Inhaltsverzeichnis zu „Cell-Based Assays for High-Throughput Screening “
Chapter 1: Introduction: Cell-based Assays for High-throughput ScreeningW. Frank An and Nicola J. Tolliday
Chapter 2: High-throughput Screening of Model Bacteria
Soumaya Zlitni, Jan E. Blanchard, and Eric D. Brown
Chapter 3: Cell-based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells
Michael R. Wyler, Deborah H. Smith, Eftihia Cayanis, Udo Toebben, Nathalie Aulner, and Thomas Mayer
Chapter 4: Large-scale Small-molecule Screen Using Zebrafish Embryos
Charles C. Hong
Chapter 5: Whole-animal High-throughput Screens: The C. elegans Model
Eyleen J. O'Rourke, Annie L. Conery, and Terence I. Moy
Chapter 6: Whole-organism Screening: Plants
April Agee and David Carter
Chapter 7: Fluorescence-based Assays
W. Frank An
Chapter 8: Reporter-gene Assays
Andy M. F. Liu, David C. New, Rico K. H. Lo, and Yung H. Wong
Chapter 9: Screening for Chemical Inhibitors of Heterologous Proteins Expressed in Yeast Using a Simple Growth-restoration Assay
Aruna D. Balgi and Michel Roberge
Chapter 10: Assay for Isolation of Inhibitors of Her2-kinase Expression
Gabriela Chiosis and Adam B. Keeton
Chapter 11: ImageJ: A Primer
Katherine Luby-Phelps
Chapter 12: High-content Screening: Flow Cytometry Analysis
Bruce S. Edwards, Susan M. Young, Irena Ivnitsky-Steele, Richard D. Ye, Eric R. Prossnitz, and Larry A. Sklar
Chapter 13: High-throughput Real-time PCR for Detection of Gene-expression Levels
Bridget K. Wagner and Zoltan Arany
Chapter 14: Interpretation of Uniform-well Readouts
Serene Josiah
Chapter 15: Extracting Rich Information from Images
Anne E. Carpenter
Bibliographische Angaben
- 2009, X, 218 Seiten, Maße: 19,8 x 26,6 cm, Gebunden, Englisch
- Herausgegeben: Paul A. Clemons, Nicola J. Tolliday, Bridget K. Wagner
- Verlag: Springer, Berlin
- ISBN-10: 1603275444
- ISBN-13: 9781603275446
- Erscheinungsdatum: 28.04.2009
Sprache:
Englisch
Rezension zu „Cell-Based Assays for High-Throughput Screening “
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Pressezitat
From the reviews: "'Cell-based assays for high-throughput screening' is a highly practical resource, written by experts in each of the techniques ... . very useful from a practical point of view, and anyone looking to find a new assay system will find a wealth of ideas here. ... aimed at molecular biologists, many other scientists - such as natural product chemists looking for new lead molecules - should find it equally useful." (Elizabeth Williamson, Phytotherapy Research, September, 2009)
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