Cellular Programming and Reprogramming

Methods and Protocols (Sprache: Englisch)

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Klappentext zu „Cellular Programming and Reprogramming “

Before the therapeutic potential of cell replacement therapy or the development of therapeutic drugs for stimulating the body's own regenerative ability to repair cells damaged by disease and injury can be fully realized, control of stem cell fate, immuno-rejection, and limited cell sources must be overcome. In Cellular Programming and Reprogramming: Methods and Protocols, expert researchers cover the most recent technologies and their related mechanisms involved in the programming and reprogramming of cell fate. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes to highlight tips on troubleshooting and avoiding known pitfalls. Essential and cutting-edge, Cellular Programming and Reprogramming: Methods and Protocols promises to aid scientists attempting to advance stem cell biology in order to better treat devastating human diseases, including cardiovascular disease, neurodegenerative disease, musculoskeletal disease, diabetes, and cancer.

Before the therapeutic potential of cell replacement therapy or the development of therapeutic drugs for stimulating the body's own regenerative ability to repair cells damaged by disease and injury can be fully realized, control of stem cell fate, immuno-rejection, and limited cell sources must be overcome. In Cellular Programming and Reprogramming: Methods and Protocols, expert researchers cover the most recent technologies and their related mechanisms involved in the programming and reprogramming of cell fate. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes to highlight tips on troubleshooting and avoiding known pitfalls.

Essential and cutting-edge, Cellular Programming and Reprogramming: Methods and Protocols promises to aid scientists attempting to advance stem cell biology in order to better treat devastating human diseases, including cardiovascular disease, neurodegenerative disease, musculoskeletal disease, diabetes, and cancer.

Inhaltsverzeichnis zu „Cellular Programming and Reprogramming “

1. Human Embryonic Stem Cell Derivation, Maintenance, and Differentiation to TrophoblastGe Lin, Kristen Martins-Taylor, and Ren-He Xu2. Isolation and Maintenance of Mouse Epiblast Stem CellsJosh G. Chenoweth and Paul J. Tesar3. Functional Assays for Hematopoietic Stem Cell Self-RenewalJohn M. Perry and Linheng Li4. Isolation Procedure and Characterization of Multipotent Adult Progenitor Cells (MAPC) from Rat Bone MarrowKartik Subramanian, Martine Geraerts, Karen A. Pauwelyn, Yonsil Park, D. Jason Owens, Manja Muijtjens, Fernando Ulloa-Montoya, Yeuhua Jiang, Catherine M. Verfaillie, and Wei-Shou Hu5. Generation of Functional Insulin-Producing Cells from Human Embryonic Stem Cells In vitroYan Shi6. Mesoderm Cell Development from ES CellsTakumi Era7. Directed Differentiation of Red Blood Cells from Human Embryonic Stem CellsShi-Jiang Lu, Qiang Feng, Jennifer S. Park, and Robert Lanza8. Directed Differentiation of Neural and Subtype Specific Neuronal Cells from hESCsBao-Yang Hu and Su-Chun Zhang9. Directing Human Embryonic Stem Cells to a Retinal FateDeepak Lamba, Julianne Gust, and Thomas A. Reh10. Bovine Somatic Cell Nuclear TransferPablo J. Ross and Jose B. Cibelli11. Cell Fusion Induced ReprogrammingJeong Tae Do and Hans R. Schöler12. An Improved Method for Generating and Identifying Human Induced Pluripotent Stem CellsPrashant Mali, Zhaohui Ye, Bin-Kuan Chou, Jonathan Yen, and Linzhao Cheng13. Using Small Molecules to Improve Generation of Induced Pluripotent Stem Cells from Somatic CellsCaroline Desponts and Sheng Ding14. Reprogramming of Committed Lymphoid Cells by Enforced Transcription Factor ExpressionHuafeng Xie, Catherine V. Laiosa, andThomas Graf15. Reprogramming of B CellsCésar Cobaleda16. Adult Cell Fate Reprogramming: Converting Liver to PancreasIrit Meivar-Levy and Sarah Ferber17. In vitro Reprogramming of Pancreatic Cells to HepatocytesDaniel Eberhard, Kathy O'Neill, Zoë D. Burke, and David Tosh18. Generation of Novel Rat and Human Pluripotent

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Stem Cells by Reprogramming and Chemical ApproachesWenlin Li and Sheng Ding19. Small Molecule Screen in Zebrafish and HSC ExpansionEirini Trompouki and Leonard I. Zon20. Zebrafish Small Molecule Screen in Reprogramming/Cell Fate ModulationKathleen M. Munson and Jing-Ruey J. Yeh

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Bibliographische Angaben

2010, 336 Seiten, 4 farbige Abbildungen, 50 Schwarz-Weiß-Abbildungen, Maße: 18,6 x 26,3 cm, Gebunden, Englisch
Verlag: Humana Press
ISBN-10: 1607616904
ISBN-13: 9781607616900