Chromosomal Mutagenesis

Contents Preface Contributors Biallelic Gene Knockouts in Chinese Hamster Ovary Cells Naoko Yamane-Ohnuki, Kazuya Yamano, and Mitsuo Satoh Highly Proficient Gene Targeting by Homologous Recombination in the Human pre-B Cell Line Nalm-6 Noritaka Adachi, Aya Kurosawa, and Hideki Koyama Robust Cell Line Development Using Meganucleases Jean-Pierre Cabaniols and Frédéric Pâques Design and Testing of Zinc Finger Nucleases for Use in Mammalian Cells Matthew Porteus Gene Targeting in Drosophila and C. elegans with Zinc-finger Nucleases Dana Carroll, Kelly J. Beumer, J. Jason Morton, Ana Bozas and Jonathan K. Trautman Orpheus Recombination: A Comprehensive Bacteriophage System for Murine Targeting Vector Construction by Transplacement Knut Woltjen, Kenichi Ito, Teruhisa Tsuzuki, and Derrick E. Rancourt Transposon Mediated Mutagenesis in Somatic Cells: Identification of transposon-genomic DNA junctions David A. Largaespada and Lara S. Collier Insertional Mutagenesis of the Mouse Germline with Sleeping Beauty Transposition Junji Takeda, Zsuzsanna Izsvák, and Zoltán Ivics Conditional Gene Trapping Using the FLEx System Thomas Floss and Frank Schnütgen Steps Towards Targeted Insertional Mutagenesis with Class II Transposable Elements Sareina Chiung-Yuan Wu, Kommineni J. Maragathavally, Craig J. Coates and Joseph M. Kaminski Targeting integration of the Saccharomyces Ty5 retrotransposon Troy L. Brady, Clarice L. Schmidt and Daniel F. Voytas Site-Specific Chromosomal Integration Mediated by f C31 Integrase Annahita Keravala and Michele P. Calos Triplex-Mediated Gene Modification Erica B. Schleifman, Joanna Y. Chin, and Peter M.Glazer Allelic exchange of unmarked mutations in Mycobacterium tuberculosis. Martin S. Pavelka, Jr. Mycobacterial Recombineering Julia C. van Kessel and Graham F. Hatfull Chromosomal Engineering of Clostridium perfringens Using Group II Introns Phalguni Gupta and Yue Chen

From the reviews:"I looked at Chromosomal Mutagenesis from the perspective of a geneticist ... . it serves as a potentially useful text. ... well written and protocols appear very detailed with good explanation that goes well beyond what one can glean from most published papers. ... On the whole, for anyone either thinking of moving into a field where chromosomal mutagenesis is not so straightforward, or looking for ideas by learning how others have solved related problems, this book could serve as a very useful resource." (Alistair Goldman, Microbiology Today, May, 2008)