Clinical Proteomics

Contents Preface...................................................................................................................................... Contributors.............................................................................................................................. 1 Overview and Introduction to Clinical Proteomics Young-Ki Paik, Hoguen Kim, Eun-Young Lee, Min-Seok Kwon and Sang Yun Cho..................... Specimen Collection for Clinical Proteomics 2 Specimen collection and handling: Standardization of blood sample collection Harald Tammen................................................................................................................................ 3 Tissue Sample Collection for Proteomics Analysis Jose L Diaz, Lisa H Cazares and O. John Semmes .......................................................... Clinical Proteomics by 2DE and direct MALDI/SELDI MS profiling 4 Protein profiling of Human Plasma Samples by Two-Dimensional Electrophoresis Sang Yun Cho, Eun-Young Lee, Hye-Young Kim, Min-Jung Kang, Hyoung-Joo Lee, Hoguen Kim and Young-Ki Paik ......................................................................................... 5 Analysis of Laser Capture Microdissected Cells by 2-Dimensional Gel Electrophoresis Daohai Zhang and Evelyn Siew-Chuan Koay ........................................................................... 6 Optimizing the Difference Gel Electrophoresis (DIGE) technology David B. Friedman and Kathryn S. Lilley .......................................................................... 7 MALDI/SELDI protein profiling of serum for the identification of cancer biomarkers Lisa H Cazares, Jose I Diaz, Rick R Drake and O John Semmes.............................................. 8 Urine sample preparation and protein profiling by two-dimensional electrophoresis and matrix assisted laser desorption ionization time-of-flight mass spectrometry. Panagiotis G. Zerefos and A. Vlahou

From the reviews:"Clinical proteomics is a fledgling field that is struggling to define its standards and whose underlying technologies continue to evolve at a very rapid pace. ... This multiauthored book provides state-of-the-art information and practical protocols on these topics and many other related areas. ... provides a wealth of background information and underlying principles. ... Overall, this is an excellent book whose natural readership includes both newcomers to the field and seasoned biomarker hunters who wish to update their knowledge or explore new techniques." (Marco Crescenzi, Annali dell'Istituto Superiore di Sanita, Vol. 44 (3), November, 2008)