Epigenome Editing

Development of locus-directed editing of the epigenome from basic mechanistic engineering to first clinical applications.- Designing epigenome editors: Considerations of biochemical and locus specificities.- Fine-Tuning the Epigenetic Landscape: Chemical Modulation of Epigenome Editors.- Using high-throughput measurements to identify principles of transcriptional and epigenetic regulators.- Reader-Effectors as actuators of epigenome editing.- Neuroepigenetic editing.- Optimized protocol for the regulation of DNA methylation and gene expression using modified dCas9-SunTag platforms.- Protocol for DNA methylation editing of imprinted loci and assessment of the effects.- Protocol for allele specific epigenome editing using CRISPR/dCas9.- Plant epigenetic editing to analyze the function of histone modifications in gene-specific regulation.- Protocol for efficient generation of chimeric antigen receptor T cells with multiplexed gene silencing by epigenome editing.- Fluorescent reporter systems to investigate chromatin effector proteins in living cells: Applications and protocol.- Plasmid delivery and single-cell plasmid expression analysis for CRISPR/dCas9-based epigenetic editing.