G Protein Coupled Receptor-Protein Interactions
(Sprache: Englisch)
G Protein Coupled Receptor-Protein Interactions examines how GPCRs organize signal transduction and control intracellular activities. Chapters written by recognized experts in each respective topic offer state-of-the-art updates on principles and technology...
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G Protein Coupled Receptor-Protein Interactions examines how GPCRs organize signal transduction and control intracellular activities. Chapters written by recognized experts in each respective topic offer state-of-the-art updates on principles and technology within this discipline, as well as stepwise protocols for methods currently applied to the analysis of protein-receptor interactions.
Klappentext zu „G Protein Coupled Receptor-Protein Interactions “
G Protein Coupled Receptor-Protein Interactions examines how GPCRs organize signal transduction and control intracellular activities. Chapters written by recognized experts in each respective topic offer state-of-the-art updates on principles and technology within this discipline, as well as stepwise protocols for methods currently applied to the analysis of protein-receptor interactions.
Inhaltsverzeichnis zu „G Protein Coupled Receptor-Protein Interactions “
- Series Preface.- Preface.
- Contributors.
- Foreward.
1. Purification of the G Protein-Coupled Receptor Rhodopsin for Structural Studies (David Salom, Ning Li, Li Zhu, Izabela Sokal and Krzysztof Palczewski).
2. Cooperativity in Ligand Binding to Deduce Receptor/Receptor Interaction (Meritxell Vivo and Philip G. Strange).
3. Analysis of Receptor-Receptor Interaction Using Time-Resolved Fluorescence Resonance Energy Transfer (Douglas Ramsay and Graeme Milligan).
4. Real Time Imaging of GPCR-medicated Arresting Translocation as a Strategy to Evaluate Receptor-Protein Interactions (Robert H. Oakley, Larry S. Barak and Marc G. Caron).
5. Localization of G Protein Coupled Receptors and Interacting Proteins in Membrane Microdomains (K. Saidas Nair, Vijaya Narayanan and Vladlen Z. Slepak).
6. Identification of Peptide Ligand Binding Domains Within G Protein-Coupled Receptors Using Photoaffinity Labeling (Maoqing Dong and Laurence J. Miller).
7. Analysis in Real-Time of the Assembly and Disassembly of Soluble G Protein-Coupled Receptor-G Protein Complexes (Seam M. Biggs, Peter I. Simons, Anna Waller, Terry Founts, Tione Buranda, Eric R. Prossnitz and Larry A. Sklar).
8. Approaches to Studying Membrane Protein Oligomerization: Use of Cysteine Cross-Linking Strategies to Analyze Receptor-Receptor Interactions (Jonathan A. Javitch, Wen Guo and Marisa E. Ring).
9. Co-Immunoprecipitation as a Strategy to Evaluate Receptor-Receptor or Receptor-Protein Interactions (Randy A. Hall).
10. Use of MALDI-TOF and Mass Spectrometry in the Evaluation of Receptor-Protein Interactions (John R. Raymond, Henry Ayiku, Justin Turner, Andrew Gelasco and John M. Arthur).
11. Use of the Yeast Two-Hybrid System to Detect Receptor-Protein Interactions (Ridwan Lin, Nadine Kabbani, Alicia Binda, Victor Canfield and Robert Levenson).
12. GST Fusion Protein and Pull-down Strategies to Evaluate Receptor-Receptor or Receptor-Protein Interactions (Frank J.S. Lee and Fang Liu).
13.
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G-Protein-Coupled Fusion to Monitor Integral Membrane Protein-Protein Interactions (Henrik Dohlman and Kathleen Ehrhard).
14. Enzyme-Linked Immunosorbent Assays as Tools in Studying Interactions of G-Protein-Coupled Receptors (Torsten Schöneberg, Katrin Sangkuhl, Holger Römpler and Angela Schulz).
- Electrophysiological Characterization of GPCR and Ligand-Gated Channel Crosstalk (Tak Pan Wong, Xian-Ming Yu and Yu Tian Wang).
14. Enzyme-Linked Immunosorbent Assays as Tools in Studying Interactions of G-Protein-Coupled Receptors (Torsten Schöneberg, Katrin Sangkuhl, Holger Römpler and Angela Schulz).
- Electrophysiological Characterization of GPCR and Ligand-Gated Channel Crosstalk (Tak Pan Wong, Xian-Ming Yu and Yu Tian Wang).
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Autoren-Porträt von David R. Sibley
SUSAN R. GEORGE, PhD, MD, FRCP, FACP, is Professor in the Departments of Medicine and Pharmacology at the University of Toronto and a Section Head of Molecular Pharmacology at the Centre for Addiction and Mental Health, Toronto.BRIAN F. O'DOWD, PhD, is Professor in the Department of Pharmacology at the University of Toronto and a Section Head of Molecular Pharmacology at the Centre for Addiction and Mental Health, Toronto.
Bibliographische Angaben
- Autor: David R. Sibley
- 2005, 1. Auflage, 320 Seiten, Maße: 18 x 25,8 cm, Gebunden, Englisch
- Herausgegeben: Susan R. George, Brian F. O'Dowd
- Verlag: Wiley & Sons
- ISBN-10: 0471235466
- ISBN-13: 9780471235460
Sprache:
Englisch
Pressezitat
"...a good starting point for the investigation of complex GPCR-protein interactions, and covers a lot of ground by introducing many different methodologies." ( E-STREAMS , September 2005)
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