Molecular Biomethods Handbook
(Sprache: Englisch)
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at...
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Klappentext zu „Molecular Biomethods Handbook “
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors' own research programs.
Inhaltsverzeichnis zu „Molecular Biomethods Handbook “
Molecular Biomethods Handbook 2nd EdContentsPART A: NUCLEIC ACID BIOMETHODS1 The manipulation of nucleic acids: basic tools and techniques - Gayle Corkill andRalph Rapley2 Restriction Enzymes - Gareth J.S. Jenkins3 Principles and Medical Applications of the Polymerase Chain Reaction - Birmal D.M.Theophilus4 Probe Design, Production, and Applications - Marilena Aquino de Muro5 Southern Blotting as a Diagnostic Method - Bronwen Harvey and Pirkko Soundy6 Introduction to Capillary Electrophoresis of DNA - Beatriz Sanchez-Vega7 Denaturing High-Performance Liquid Chromatography (DHPLC) for nucleic acidanalysis - Kim Hung Leung and Shea Ping Yip8 Denaturing Gradient Gel Electrophoresis (DGGE) - Jeroen H. Roelfsema and DorienJ.M. Peters9 Single Strand Conformation Polymorphism (SSCP) Analysis - Kim Hung Leung andShea Ping Yip10 Randomly amplified polymorphic DNA (RAPD); a useful tool for genomiccharacterization of different organisms - Lúcia Maria da Cunha Galvão and ElianeLages-Silva11 Quantification of mRNA using Real Time RT-PCR - the SYBR solution - DavidSugden and Patricia de Winter12 Quantitative Analysis of DNA Sequences by PCR and Solid Phase Sequencing - AnuSuomalainen and Ann-Christine Syvanen13 MAPH (Multiplex Amplifiable Probe Hybridization) - Carolina Sismani, LudmilaKousoulidou and Philippos C. Patsalis14 Gene Expression Profiling - Arnis Druka, Robbie Waugh and Pete Hedley15 Comparative Genomic Hybridization in Clinical and Medical Research - Peng-HuiWang, Yann-Jang Chen and Chi-Hung Lin16Subtractive Hybridization - Craig Winstanley17 Fluorescence in situ Hybridization - Jane Bayani and Jeremy A. Squire18 Quantitative Trait Locus Mapping to Identify Genes for Complex Traits in Mice -Jonathan D. Smith19 cDNA Microarrays - Phillip G. Febbo20 Mapping Techniques - Simon G. Gregory21 Single Nucleotide Polymorphisms Overview - Nameeta Shah22 Bioinformatics Tools for Gene and Protein Sequence Analysis- Bernd H.A. Rehm andFrank ReineckePART B: PROTEIN AND CELL
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METHODS23. Protein Electrophoresis - David Sheehan and Siobhan O'Sullivan24. Protein blotting - Patricia Gravel25. Capillary electrophoresis of proteins - Mark Strege26. Autoradiography and fluorography - Bronwen Harvey27. Mass spectrometry of proteins & peptides - Kenneth G. Standing28. Post-translational modifications - Christoph Kannicht and Birte Fuchs29. Protein microarray technology - Charlotte H. Clarke and Eric T. Fung30. Protein-protein interactions - Hae Ryoun Park, Lisa Montoya Cockrell, Yuhong Du, Andrea Kasinski, Jonathan Havel, Jing Zhao, Francisca Reyes-Turcu, Keith Wilkinson and Haian Fu31. Glycoprotein analysis - Terry Butters and David C.A. Neville32. Solid Phase Peptide Synthesis - Mare Cudic and Gregg B. Fields33. Monoclonal antibodies - Zhong J. Zhang and Maher Albitar34. Antibody Phage display - Rob Aitken35. Protein engineering - Thomas Willemsen, Urs B. Hagemann,Eva M. Jouaux, Sabine Stebel, Jody M. Mason, Kristian M. Müller and Katja M. Arndt36. Directed protein evolution - Sabine C. Stebel, Annette Gaida, Katja M. Arndt and Kristian M. Müller37. Enzyme Linked Immunosorbent Assay - John Crowther38. Epitope mapping - Glenn E. Morris39.
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Bibliographische Angaben
- 2nd ed. 2008, 1124 Seiten, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Ralph Rapley, John M. Walker
- Verlag: Humana Press
- ISBN-10: 1603273700
- ISBN-13: 9781603273701
- Erscheinungsdatum: 25.08.2008
Sprache:
Englisch
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