mRNA Processing
Methods and Protocols
(Sprache: Englisch)
This detailed volume assembles a number of the most commonly used and state-of-the-art methodologies in the revolutionary field of mRNA processing in order to aid researchers in choosing the best method for their specific problems. Given that the vast...
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This detailed volume assembles a number of the most commonly used and state-of-the-art methodologies in the revolutionary field of mRNA processing in order to aid researchers in choosing the best method for their specific problems. Given that the vast majority of alternative mRNA processing events has yet to be explored, these techniques promise to breathe new life into the myriad new ideas currently underway. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. <div> </div><div>Practical and easy to use, mRNA Processing: Methods and Protocols seeks to aid scientists venturing into what's certain to be another explosive period of growth in this dynamic field of study.</div>
Inhaltsverzeichnis zu „mRNA Processing “
1. Detection and Identification of Uncapped RNA by Ligation-Mediated Reverse Transcription Polymerase Chain Reaction C. Kiong Ho
2. Preparation of Splicing Competent Nuclear Extract from Mammalian Cells and In Vitro Pre-mRNA Splicing Assay
Maliheh Movassat, Hossein Shenasa, and Klemens J. Hertel
3. Single-Molecule Analysis of Pre-mRNA Splicing with Colocalization Single-Molecule Spectroscopy (CoSMoS) Joerg E. Braun and Victor Serebrov
4. In Vitro Modulation of Endogenous Alternative Splicing Using Splice-Switching Antisense Oligonucleotides
Jeong Eun Park and Luca Cartegni
5. Purification of mRNA Processing Complexes Using an RNA Affinity Approach
Xiuye Wang and Yongsheng Shi
6. Polyadenylation Site-Based Analysis of Transcript Expression by 3'READS+
Dinghai Zheng and Bin Tian
7. Comprehensive Identification of mRNA Polyadenylation Sites by PAPERCLIP
Hun-Way Hwang and Robert B. Darnell
8. Microinjection and Fluorescence In Situ Hybridization Assay for Studying mRNA Export in Mammalian Cells
Ke Wang, Min Shi, and Hong Cheng
9. Methods for the Detection of Adenosine-to-Inosine Editing Events in Cellular RNA
... mehr
Eimile Oakes, Pranathi Vadlamani, and Heather A. Hundley
10. Using RNA-Seq to Discover Genetic Polymorphisms that Produce Hidden Splice Variants
Shayna Stein, Emad Bahrami-Samani, and Yi Xing
11. Inducible Expression of Eukaryotic Circular RNAs from Plasmids
Deirdre C. Tatomer, Dongming Liang, and Jeremy E. Wilusz
12. Methods for Extraction of RNA, Proteins, or Protein Complexes from Subcellular Compartments of Eukaryotic Cells
Kyu-hyeon Yeom and Andrey Damianov
13. Isolation of Newly Transcribed RNA Using the Metabolic Label 4-Thiouridine
Angela Garibaldi, Francisco Carranza, and Klemens J. Hertel
14. Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-End Crosslinking and Immunoprecipitation (seCLIP) Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, and Gene W. Yeo
15. Purification of Transcript-Specific mRNP Complexes Formed In Vivo from Saccharomyces cerevisiae
Jenna E. Smith and Kristian E. Baker
16. A Cell-Based High-Throuput Method for Identifying Modulators of Alternative Splicing
Sika Zheng
17. Genome-Wide RNAi Screens for RNA Processing Events in Drosophila Melanogaster S2 Cells
Natoya J. Peart and Eric J. Wagner
18. Assaying RNA Structure Inside Living Cells with SHAPE
Chao Feng, Dalen Chan, and Robert C. Spitale
Eimile Oakes, Pranathi Vadlamani, and Heather A. Hundley
10. Using RNA-Seq to Discover Genetic Polymorphisms that Produce Hidden Splice Variants
Shayna Stein, Emad Bahrami-Samani, and Yi Xing
11. Inducible Expression of Eukaryotic Circular RNAs from Plasmids
Deirdre C. Tatomer, Dongming Liang, and Jeremy E. Wilusz
12. Methods for Extraction of RNA, Proteins, or Protein Complexes from Subcellular Compartments of Eukaryotic Cells
Kyu-hyeon Yeom and Andrey Damianov
13. Isolation of Newly Transcribed RNA Using the Metabolic Label 4-Thiouridine
Angela Garibaldi, Francisco Carranza, and Klemens J. Hertel
14. Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-End Crosslinking and Immunoprecipitation (seCLIP) Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, and Gene W. Yeo
15. Purification of Transcript-Specific mRNP Complexes Formed In Vivo from Saccharomyces cerevisiae
Jenna E. Smith and Kristian E. Baker
16. A Cell-Based High-Throuput Method for Identifying Modulators of Alternative Splicing
Sika Zheng
17. Genome-Wide RNAi Screens for RNA Processing Events in Drosophila Melanogaster S2 Cells
Natoya J. Peart and Eric J. Wagner
18. Assaying RNA Structure Inside Living Cells with SHAPE
Chao Feng, Dalen Chan, and Robert C. Spitale
... weniger
Bibliographische Angaben
- 2017, 1st ed. 2017, XI, 258 Seiten, 33 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Yongsheng Shi
- Verlag: Springer, Berlin
- ISBN-10: 1493972030
- ISBN-13: 9781493972036
- Erscheinungsdatum: 02.08.2017
Sprache:
Englisch
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