NMR for Chemists and Biologists

1. The basis of Nuclear Magnetic Resonance Spectroscopy1.1. Introduction1.2. Physical principles of NMR spectroscopy1.2.1. The basis of NMR spectroscopy: a vector approach1.2.2. The basis of NMR spectroscopy: a naïve quantum approach1.2.3. The nuclei in NMR1.3. Spin relaxation1.3.1. Spin-lattice relaxation time1.3.2. Spin-spin relaxation time1.3.3. Sources of variation in local fields1.4. Pulse techniques1.4.1. How a pulse works: the time and frequency domains1.4.2. Multipulse experiments: measurement of the T1- and T2-relaxation times as examples1.5. Practical aspects of NMR1.5.1. The magnet1.5.2. The probe1.5.3. The lock-system1.5.4. The transmitter/receiver system: quadrature detection1.5.5. The shim system1.5.6. Pulse field gradients1.5.7. Sample preparation1.6. References

2. Spectroscopic parameters in Nuclear Magnetic Resonance2.1. The chemical shift and the spectral intensity2.1.1. The shielding screening constant2.1.2. The chemical shift2.1.3. Signal intensity2.2. The scalar coupling constant2.2.1. Spin-spin coupling2.2.2. How does spin-spin coupling occur?2.2.3. Variations in the value of J2.2.4. Spin-spin decoupling2.3. The nuclear Overhauser effect2.3.1. The basis of the NOE: a two spin system2.3.2. NOEs in multi-spin systems2.4. References  

3. Basic NMR experiments3.1. Introduction3.2. 1D NMR 3.2.1. Sensitivity and frequency3.2.2. Acquisition and processing3.2.3. 1D spectra of 1H, 13C,31P and 19F3.3. Multidimensional NMR3.3.1. Generating dimensions in NMR3.3.2. 2D data acquisition and processing 3.4. Homonuclear shift correlation: correlations through the chemical bond3.4.1. COSY. Experiment interpretation and practical aspects3.4.2. TOCSY. Practical aspects 3.4.3. Correlation for diluted spins: the INADEQUATE experiment. Double-quantum selection3.5. Heteronuclear shift correlation: correlations through the chemical bond3.5.1. Polarization transfer experiments: SPT and INEPT sequences; indirect spectroscopy3.5.2. Heteronuclear single