Nucleases
Molecular Biology and Applications
(Sprache: Englisch)
This book presents a current survey or nucleases along with up-to-date applications oriented towards laboratory utility in recombinant DNA technology, molecular cloning, and genomics. It provides guidance to understanding how nucleases can be exploited to...
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This book presents a current survey or nucleases along with up-to-date applications oriented towards laboratory utility in recombinant DNA technology, molecular cloning, and genomics. It provides guidance to understanding how nucleases can be exploited to make new products involving molecular cloning and biotechnology as well as for diagnostic purposes.
Klappentext zu „Nucleases “
* Provides detailed applications of nucleases in recombinant DNA technology, molecular cloning, biotechnology, pharmaceuticals, and commerce.* Covers the role of nucleases in biological systems, with focus on understanding their role in causing human diseases.
Inhaltsverzeichnis zu „Nucleases “
Preface.List of Nobel Prize Winners for Their Research Work with Nucleases.
About the Author.
1. Introduction.
I. Historical Perspectives.
II. Protein, RNA, DNA, and Other Molecules as Nucleases.
III. Nature of Enzymatic Reactions Catalyzed by Nucleases.
IV. Classification.
A. Nature of Substrates.
B. Mode of Attack.
C. Site-Specificity and Structure-Selectivity.
V. Methods for the Study of Nucleases.
A. Methods for the Assay of the Enzymatic Activity.
B. Methods for the Study and Characterization of Nucleases.
VI. Genetics of Nucleases and Biological Roles.
VII. Applications of Nucleases.
2. Ribonuclease.
I. General Ribonucleases.
A. Microbial Ribonucleases.
1. RNaseT1.
2. RNaseT2.
B. Mammalian Ribonucleases.
1. Bovine Pancreatic Rnase.
2. RNaseA.
3. Human Pancreatic Ribonuclease (HPR).
4. Human Nonsecretory Ribonuclease (HNSR).
5. Human Major Basic Protein (MBP), Eosinophil Cationic Protein (ECP) and Eosinophil-Derived Neurotoxin (EDN).
6. Angiogenin.
7. Interferon-Induced Mammalian Ribonuclease.
8. Human RNase with a Possible Role in Tumor Suppression.
C. Plant Ribonucleases.
D. Evolution of Ribonucleases.
II. Ribonucleases Involved in RNA Processing (Trimming, Splicing, and Editing).
A. RNaseIII and RNaseIII-Like Enzymes.
B. RNaseP.
C. RNaseE.
D. RNaseM5.
E. RNaseD.
F. Eukaryotic RNA-Splicing Enzymes.
1. Yeast tRNA Splicing Endonuclease.
III. Ribonuclease H.
A. E. coli RNaseH.
B. Retroviral Reverse Transcriptase RNaseH.
C. Yeast RNaseH.
D. Human RNaseH.
E. Other Eukaryotic RNaseH.
F. Biological Function of RNaseH.
IV. Proofreading Activity of RNA Polymerase.
3. Deoxyribonuclease.
I. Classification of Enzymes.
A. Deoxyribonucleases.
B. Endonucleases
C. Exonuclease.
II. Properties of Enzymes from Different Organisms.
A. Bacterial Enzymes.
1. Exonuclease I.
2. Exonuclease II.
3. Exonuclease
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III.
4. Application of the Enzyme Exonuclease III.
5. Exonucleases IVA and IVB.
6. Exonuclease V (RecBCD Enzyme).
7. RecBCD (Exo V) from Other Organisms.
8. Exonuclease VI.
9. Exonuclease VII.
10. Exonucleases Associated with DNA Polymerases.
11. Exonuclease VIII.
B. Endonucleases.
1. Bacterial Enzymes.
2. Mammalian Deoxyribonuclease.
4. Restriction Endonucleases.
I. Occurrence, Classification, and Their General Properties.
A. Different Restriction Endonucleases and Their Properties.
II. Type I Restriction Endonucleases.
A. Purification and General Properties.
B. Recognition Sequences and Nature of Substrate.
C. Different Kinds of Type II Restriction Endonucleases.
D. Genetics.
E. Cleavage Mechanism.
III. Type II Restriction Endonucleases.
A. Enzyme Purification and Assay.
B. General Properties of the Enzyme.
C. Crystal Structure of the Restriction Endonucleases.
D. Reaction Conditions and Enzyme Specificity.
E. Nature of Substrate.
1. Synthetic Oligonucleotides.
2. DNA with Base Analogs.
3. Methylated DNA.
4. Single-Stranded DNA.
5. DNA-RNA Hybrids as Substrate.
F. Inhibition of Restriction Endonucleases.
G. Restriction Endonuclease Genes.
IV. Type III Restriction Endonucleases.
V. Evolutionary Significance and Biological Role.
VI. Application of Restriction Nucleases.
VII. General Tips for Beginners or the First-Time Users of Restriction Enzymes.
5. Damage-Specific Nucleases.
I. Classification and Assay .
A. AP Endonucleases.
B. Enzymes that Directly Attack Phosphodiester Linkages in the Damaged DNA Region.
C. Assay.
II. Properties of Two Groups of Enzymes from Different Organisms.
A. AP Endonucleases.
1. AP Endonucleases Associated with DNA Glycosylase Activity.
2. M. luteus Enzyme.
3. E. coli Endonuclease III.
B. AP Endonuclease Associated with Other Enzyme Activities.
1. E. coli Exonuclease III AP-Endonuclease Activity.
4. Application of the Enzyme Exonuclease III.
5. Exonucleases IVA and IVB.
6. Exonuclease V (RecBCD Enzyme).
7. RecBCD (Exo V) from Other Organisms.
8. Exonuclease VI.
9. Exonuclease VII.
10. Exonucleases Associated with DNA Polymerases.
11. Exonuclease VIII.
B. Endonucleases.
1. Bacterial Enzymes.
2. Mammalian Deoxyribonuclease.
4. Restriction Endonucleases.
I. Occurrence, Classification, and Their General Properties.
A. Different Restriction Endonucleases and Their Properties.
II. Type I Restriction Endonucleases.
A. Purification and General Properties.
B. Recognition Sequences and Nature of Substrate.
C. Different Kinds of Type II Restriction Endonucleases.
D. Genetics.
E. Cleavage Mechanism.
III. Type II Restriction Endonucleases.
A. Enzyme Purification and Assay.
B. General Properties of the Enzyme.
C. Crystal Structure of the Restriction Endonucleases.
D. Reaction Conditions and Enzyme Specificity.
E. Nature of Substrate.
1. Synthetic Oligonucleotides.
2. DNA with Base Analogs.
3. Methylated DNA.
4. Single-Stranded DNA.
5. DNA-RNA Hybrids as Substrate.
F. Inhibition of Restriction Endonucleases.
G. Restriction Endonuclease Genes.
IV. Type III Restriction Endonucleases.
V. Evolutionary Significance and Biological Role.
VI. Application of Restriction Nucleases.
VII. General Tips for Beginners or the First-Time Users of Restriction Enzymes.
5. Damage-Specific Nucleases.
I. Classification and Assay .
A. AP Endonucleases.
B. Enzymes that Directly Attack Phosphodiester Linkages in the Damaged DNA Region.
C. Assay.
II. Properties of Two Groups of Enzymes from Different Organisms.
A. AP Endonucleases.
1. AP Endonucleases Associated with DNA Glycosylase Activity.
2. M. luteus Enzyme.
3. E. coli Endonuclease III.
B. AP Endonuclease Associated with Other Enzyme Activities.
1. E. coli Exonuclease III AP-Endonuclease Activity.
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Autoren-Porträt von Nawin C. Mishra
NAWIN C. MISHRA, PhD, is Professor in the Department of Biological Sciences at the University of South Carolina. He was elected a Fellow of the American Association for the Advancement of Science in 1986 for his original contribution to the study of gene transfer in fungi.
Bibliographische Angaben
- Autor: Nawin C. Mishra
- 2002, 1. Auflage, 314 Seiten, Maße: 24,2 cm, Gebunden, Englisch
- Verlag: Wiley & Sons
- ISBN-10: 0471394610
- ISBN-13: 9780471394617
Sprache:
Englisch
Pressezitat
"...a useful text for students and professionals..." ( Genomics and Proteomics , May 1, 2003)
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