Practical Enzymology

INTRODUCTION ENZYME NOMENCLATURE ENZYME REACTIONS

- Theory of the enzyme reaction Michaelis-Menten equation Theory of the enzyme test Theory of coupled enzyme reactions Substrate determination Instrumental aspects Enzyme assays Diverse methods Enzyme immunoassays

BINDING MEASUREMENTS

- Irreversible, reversible, specific and unspecific binding Binding measurement by size discrimination Spectroscopic techniques Other methods for binding measurements

ENZYMES IN TECHNICAL APPLICATIONS

- Principles of enzyme immobilisation Methods for enzyme immobilisation Analysis of immobilised enzymes Enzyme reactors Biosensors Immobilized enzymes in therapy

APPENDIX

- List of enzymes according to EC numbers

Prof. Dr. Hans Bisswanger ist Emeritus am Interfakultären Institut für Biochemie in Tübingen. Er war langjähriger Mitarbeiter am Institut und hat dort eine Reihe von Kursen über Enzyme Kinetik und Technologie entwickelt und geleitet. Aufbau und Leitung eines Enzymologie-Praktikums für Biochemie-Studenten.Arbeiten an Struktur und Kinetik des Pyruvat-Dehydrogenase-Komplexes, an thermophilen Enzymen, wie der Xylose-Isomerase und an Immobilisierung von Enzymen am Beispiel verschiedener Proteasen im Rahmen eines Forschungsprojekts über die Konservierung säuregeschädigten Bibliotheksmaterials als Beispiel technischer Anwendung von Enzymen. Einrichtung und Betreuung eines deutsch-chinesischen Studentenaustausches in Zu-sammenarbeit mit einer Pekinger Universität.

Buchkurzvorstellung der ersten! Auflage mit farbigem Cover in:

Process August 2004New Drugs, November 2004

CEABA-VtB, Literaturdatenbank der Dechema

"In Summa hat der Autor mit Practical Enzymology Enzymkinetik

"We strongly recommend Bisswanger's "Practical Enzymology"to the readers of the Journal of Natural Sciences, Biology, and Medicine involved in biomedical research. We believe that this manual has the potential to become a classic of its kind provided that the author will: (a) maintain the simplicity of the writing style and the practical approach to its content in future editions and (b) build up on the provided pool of enzymatic assay protocols." ( Journal of Natural Science, Biology and Medicine , 1 July 2014)
"For the interested undergraduate, an incoming PhD student, or the experienced scientist working with enzymes for the first time, the book will be certainly worth their time." ( Bioessays , 2011)
"In Arthur Kornberg s words there is never a dull enzyme . Bisswanger s Practical Enzymology returns to a similar philosophy of enzymology where important creative research can be carried forward without necessarily needing massive research groups and hugely expensive equipment. For the interested undergraduate, an incoming PhD student, or the experienced scientist working with enzymes for the first time, the book will be certainly worth their time." ( Bioessays , 2011)

"This book would be a great resource to both undergraduate and graduate students conducting research that in any way involves enzyme-catalyzed reactions. Summing Up: Highly recommended. Upper-division undergraduates through professionals." ( Choice , 1 December 2011)

"The book could serve as a practical auxilliary to a theory-oriented textbook in an introductory course . . . . The second has been updated and corrected, color illustrations have been added to the text, and a companion website contains animations and other resources." ( Book News , 1 August 2011)