Proteomic Profiling and Analytical Chemistry

Chapter 1: Introduction Chapter 2: Biomolecules Chapter 3: Fundamental Strategies of Protein and Peptide Sample Preparation Chapter 4: Protein Extraction and Precipitation Chapter 5: Immunoaffinity Depletion of High-Abundant Proteins for Proteomic Sample Chapter 6: Gel Electrophoresis Chapter 7: Quantitative Measurements in Proteomics Chapter 8: Proteomic Database Search and Analytical Quantification Chapter 9: Microarrays and Dynamics of Fluorescent Dyes Chapter 10: Design and Statistical Analysis of Mass-Spectrometry Based Quantitative Proteomics Data Chapter 11: Principles of Analytical Validation Chapter 12: Validation in Proteomics and Regulatory Affairs

Dr. Pawel Ciborowski obtained an MS in Biochemistry from Warsaw University and a PhD in Bacteriology/Biochemistry in 1983 from the National Institute of Hygiene in Warsaw. After spending two years as the Alexander von Humboldt Fellow at the University of Cologne and two years as Visiting Scientist at the University of Lund, he came to the University of Pittsburgh. Since 2003, he has been a faculty member at the University of Nebraska Medical Center where he conducts his own research and directs the Mass Spectrometry and Proteomics Core Facility and teaches courses in biochemistry, mass spectrometry, and proteomics. He is the author of over 70 papers and reviews, as well as several contributions to books and textbooks. His research is focused on the correlation of structure and function of proteins, their receptors and the impact of posttranslational modifications on their functions in understanding molecular mechanisms of pathological processes and applications of this information in designing new strategies for disease prevention, early diagnosis, and control. Using state-of-the-art techniques in proteomics, he is investigating the structure and function of proteins which are involved in (i) response to changes in cell's environment, (ii) response to stimuli such as tissue injury, infection, drug treatment, and (iii) during malignant transformation. Dr. Jerzy Silberring obtained a PhD in Chemistry in 1978 at Jagiellonian University, Kraków, Poland. He received a Docent of Biochemistry title at the University of Uppsala in 1991, and became a full professor of biochemistry at the Jagiellonian University in 2001. He spent 10 years at the Uppsala University and Karolinska Institute in Stockholm. He is the author of over 200 papers and reviews mainly on proteolytic enzymes, neuropeptides, neurobiology of pain and drug dependence, proteomics, and mass spectrometry. His investigations have contributed to our understanding of the regulation of neuropeptides and their

"Because of the heavy emphasis on sample preparation and protein separation, the book is incredibly useful as an overview of these methodologies, specifically pointing out steps and precautions to take for optimal sample quality.This book fills a void in proteomic literature and provides a starting point for novices to understand sample preparation and pitfalls to avoid, without delving into details of how a mass spectrometer functions."-- Doody.com, November 8, 2013 "In this concise guide for researchers, technicians, and students, Ciborowski and Silberring document how to use analytical techniques to ensure precision and low rates of false positives in the fast-growing field of proteomics research."-- Reference & Research Book News, October 2013