Receptor Tyrosine Kinases
Methods and Protocols
(Sprache: Englisch)
Receptor Tyrosine Kinases: Methods and Protocols explores on the most widely studied of the protein classes, known for their central role in several cellular functions and in a variety of human pathologies. Beginning with a section on activation and...
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Receptor Tyrosine Kinases: Methods and Protocols explores on the most widely studied of the protein classes, known for their central role in several cellular functions and in a variety of human pathologies. Beginning with a section on activation and signaling of Receptor Tyrosine Kinases (RTKs), the book continues with chapters on aberrant expression of RTKs, trafficking and negative regulation, as well as RTKs as pharmacological targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Receptor Tyrosine Kinases: Methods and Protocols contains an up-to-date overview of the most relevant and widely used methods employed in this field, providing detailed protocols that molecular and cellular biologists could easily adopt in their research programs on RTKs.
Inhaltsverzeichnis zu „Receptor Tyrosine Kinases “
Part I: RTKs Activation and Signalling1. Analysis of Receptor Tyrosine Kinase (RTK) Phosphorylation by Immunoblotting
Martina McDermott and Norma O'Donovan
2. Analysis of Changes in Phosphorylation of Receptor Tyrosine Kinases: Antibody Arrays
Sweta Rani and Lorraine O'Driscoll
3. Analysis of Epidermal Growth Factor Receptor Dimerization by BS3 Crosslinking
Harmony F. Turk and Robert S. Chapkin
4. Single-Molecule Optical Methods Analyzing Receptor Tyrosine Kinase Activation in Living Cells
Inhee Chung and Ira Mellman
5. Evaluation of the Dimerization Profiles of HER Tyrosine Kinases by Time-Resolved Förster Resonance Energy Transfer (TR-FRET)
Evelyne Lopez-Crapez, Alexandre Ho-Pun-Cheung, Patrick Garnero, and Hervé Bazin
6. Applying the Proximity Ligation Assay (PLA) to Mouse Preimplantation Embryos for Identifying Protein-Protein Interactions In Situ
Ivan Bedzhov and Marc P. Stemmler
Part II: RTKs Aberrant Expression
7. Analysis of Receptor Tyrosine Kinase Gene Amplification on the Example of FGFR1
Diana Boehm, Anne von Mässenhausen, and Sven Perner
8. Quantification of the Effects of Mutations on Receptor Tyrosine Kinase (RTK) Activation in Mammalian Cells
Lijuan He and Kalina Hristova
Part III: RTKs Trafficking and Negative Regulation
9. Cell Surface Biotinylation of Receptor Tyrosine Kinases to Investigate Intracellular Trafficking
Mathieu J.F. Crupi, Douglas S. Richardson, and Lois M. Mulligan
10. Studying N-Linked Glycosylation of Receptor Tyrosine Kinases
Harry M. Itkonen and Ian G. Mills
11. Identification of Receptor Protein Tyrosine Phosphatases (RPTPs) as Regulators of Receptor Tyrosine Kinases (RTKs) Using an RPTP siRNA-RTK Substrate Screen
Hojin Lee and Anton M. Bennett
12. Downregulation of Receptor Tyrosine Kinases Through Ubiquitination: Analysis by Immunodetection
Noriaki Shimokawa and Noriyuki Koibuchi
13. Regulation of Receptor Tyrosine Kinases by miRNA:
... mehr
Overexpression of miRNA Using Lentiviral Inducible Expression Vectors
XiangDong Le, Andrew T. Huang, Yunyun Chen, and Stephen Y. Lai
Part IV: RTKs as Pharmacological Targets
14. Human Tumor Xenografts in Mouse as a Model for Evaluating Therapeutic Efficacy of Monoclonal Antibodies or Antibody-Drug Conjugate Targeting Receptor Tyrosine Kinases
Liang Feng, Wei Wang, Hang-Ping Yao, Jianwei Zhou, Ruiwen Zhang, and Ming-Hai Wang
15. Receptor Tyrosine Kinase Targeting in Multicellular Spheroids
Susan Breslin and Lorraine O'Driscoll
16. Receptor Tyrosine Kinases and Drug Resistance: Development and Characterization of In Vitro Models of Resistance to RTK Inhibitors
Claire Corcoran and Lorraine O'Driscoll
XiangDong Le, Andrew T. Huang, Yunyun Chen, and Stephen Y. Lai
Part IV: RTKs as Pharmacological Targets
14. Human Tumor Xenografts in Mouse as a Model for Evaluating Therapeutic Efficacy of Monoclonal Antibodies or Antibody-Drug Conjugate Targeting Receptor Tyrosine Kinases
Liang Feng, Wei Wang, Hang-Ping Yao, Jianwei Zhou, Ruiwen Zhang, and Ming-Hai Wang
15. Receptor Tyrosine Kinase Targeting in Multicellular Spheroids
Susan Breslin and Lorraine O'Driscoll
16. Receptor Tyrosine Kinases and Drug Resistance: Development and Characterization of In Vitro Models of Resistance to RTK Inhibitors
Claire Corcoran and Lorraine O'Driscoll
... weniger
Bibliographische Angaben
- 2014, 2015, XI, 181 Seiten, 31 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Serena Germano
- Verlag: Springer, Berlin
- ISBN-10: 1493917889
- ISBN-13: 9781493917884
- Erscheinungsdatum: 16.10.2014
Sprache:
Englisch
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