Seed Dormancy
Methods and Protocols
(Sprache: Englisch)
Understanding seed-related processes is of major social, environmental, and economic concern. The viability and vigor of seeds are the very basis for sustainable agriculture and forestry, and comprehending the molecular and cellular events underlying these...
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Understanding seed-related processes is of major social, environmental, and economic concern. The viability and vigor of seeds are the very basis for sustainable agriculture and forestry, and comprehending the molecular and cellular events underlying these processes will become increasingly important to many economical sectors and for species that provide the world's food supply. Seed Dormancy: Methods and Protocols covers analytical methods and approaches which have already lead to significant advances in the understanding of seed dormancy and germination. Chapters cover explanations of processes leading to the induction, maintenance, and termination of seed dormancy, the classification of different dormancy types, as well as an overview of protocols used for dormancy-termination of seeds of conifer species. This volume emphasizes methods essential for abscisic acid (ABA) analyses, including methods that have been important for receptor identification, analyses of ABA-catabolizing enzymes (the 8'-hydroxylases), and identification of novel signal transduction components, interacting partners, and/or response factors. The volume closes by addressing the development of new technologies, including spectroscopic methods (some of which allow for non-destructive sampling) as well as highly effective tissue-printing methods for seed dormancy research. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Seed Dormancy: Methods and Protocols features detailed methods that will prove invaluable for both applied and fundamental seed research.
Authoritative and easily accessible, Seed Dormancy: Methods and Protocols features detailed methods that will prove invaluable for both applied and fundamental seed research.
Klappentext zu „Seed Dormancy “
Understanding seed-related processes is of major social, environmental, and economic concern. The viability and vigor of seeds are the very basis for sustainable agriculture and forestry, and comprehending the molecular and cellular events underlying these processes will become increasingly important to many economical sectors and for species that provide the world's food supply. Seed Dormancy: Methods and Protocols covers analytical methods and approaches which have already lead to significant advances in the understanding of seed dormancy and germination. Chapters cover explanations of processes leading to the induction, maintenance, and termination of seed dormancy, the classification of different dormancy types, as well as an overview of protocols used for dormancy-termination of seeds of conifer species. This volume emphasizes methods essential for abscisic acid (ABA) analyses, including methods that have been important for receptor identification, analyses of ABA-catabolizing enzymes (the 8'-hydroxylases), and identification of novel signal transduction components, interacting partners, and/or response factors. The volume closes by addressing the development of new technologies, including spectroscopic methods (some of which allow for non-destructive sampling) as well as highly effective tissue-printing methods for seed dormancy research. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Seed Dormancy: Methods and Protocols features detailed methods that will prove invaluable for both applied and fundamental seed research.
Inhaltsverzeichnis zu „Seed Dormancy “
I. Introduction1. Challenges Facing the Forest Industry in Relation to Seed Dormancy and Seed Quality
Michael U. Stoehr and Yousry A. El-Kassaby
2. Challenges Facing Seed Banks and Agriculture in Relation to Seed Quality
M. Verónica Rodríguez, Peter E. Toorop, and Roberto L. Benech-Arnold
II. Breaking Seed Dormancy and Standardizing Seed Dormancy Research
3. Standardizing Seed Dormancy Research
Henk WM Hilhorst
4. Eyeing Emergence: Modified Treatments for Terminating Dormancy of Conifer Seeds
J. Allan Feurtado and Allison R. Kermode
5. Production of Seed Samples for the Effective Molecular Analysis of Dormancy Cycling in Arabidopsis
Steven Footitt and William E. Finch-Savage
III. Hormone Analyses
6. Approaches to the Identification of ABAR as an Abscisic Acid Receptor
Xiao-Fang Wang, Qi Xin, Yuan-Yue Shen, and Da-Peng Zhang
7. Profiling of Hormones and Related Metabolites in Seed Dormancy and Germination Studies
Mitsunori Seo, Yusuke Jikumaru, and Yuji Kamiya
8. In Vitro Assay for ABA 8'-Hydroxylase: Implications for Improved Assays for Cytochrome P450 Enzymes
Joan E. Krochko and Adrian J. Cutler
9. Functional Analysis of Abscisic Acid 8'-Hydroxylase
Akira Endo, Mitsuhiro Kimura, Naoto Kawakami, and Eiji Nambara
IV. Gene, RNA, and Protein Analyses
10. Isolation of Genetic Material from Arabidopsis Seeds
Urszula Piskurewicz and Luis Lopez-Molina
11. Identification and Characterization of Quantitative Trait Loci (QTL) that Control Dormancy in Arabidopsis
... mehr
Leonie Bentsink and Maarten Koornneef
12. Identification of Seed Dormancy Mutants by Activation Tagging
Tiehan Zhao, Ying Zeng, and Allison R. Kermode
13. Isolation of MicroRNAs that Regulate Seed Dormancy and Germination
Arun Kumar M.B., Ruth C. Martin, and Hiro Nonogaki
14. Proteomics and Post-Translational Proteomics of Seed Dormancy and Germination
Loïc Rajjou, Maya Belghazi, Julie Catusse, Laurent Ogé, Erwann Arc, Béatrice Godin, Kamel Chibani, Sonia Ali-Rachidi, Boris Collet, Philippe Grappin, Marc Jullien, Karine Gallardo, Claudette Job, and Dominique Job
V. Epigenetic Changes Associated with Seed Dormancy
15. Chromatin Dynamics during Seed Dormancy
Yongxiu Liu, Regina Geyer, Vittoria Brambilla, Kazumi Nakabayashi, and Wim J.J. Soppe
16. Emerging Mass Spectrometry-Based Technologies for Analyses of Chromatin Changes: Analysis of Histones and Histone Modifications
Brinda Shah, Rachel L. Kozlowski, and Christoph H. Borchers
VI. Spectroscopic and Whole Tissue Analyses
17. In Vivo Nuclear Magnetic Resonance (NMR) Metabolite Profiling in Plant Seeds
Victor Terskikh and Allison R. Kermode
18. In Vivo 1H NMR Microimaging during Seed Imbibition, Germination, and Early Growth
Victor Terskikh, Kerstin Müller, Allison Kermode, and Gerhard Leubner-Metzger
19. Tissue Printing Methods for Localization of RNA and Proteins that Control Seed Dormancy and Germination
Wioletta E. Pluskota, Kent J. Bradford, and Hiro Nonogaki
VII. Analyses of Reactive Oxygen Species, Protein Damage and Repair
20. Analyses of Reactive Oxygen Species and Antioxidants in Relation to Seed Longevity and Germination
Christophe Bailly and Ilse Kranner
21. Protein Damage and Repair Controlling Seed Vigor and Longevity
Laurent Ogé, Caroline Broyart, Boris Collet, Béatrice Godin, Denis Jallet, Gildas Bourdais, Dominique Job, and Philippe Grappin
22. Nitric Oxide Methods in Seed Biology
Paul C. Bethke, Igor Libourel, Jan Vitecek, and Russell L. Jones
VIII. Bioinformatic Resources
23. Seed Bioinformatics
George W. Bassel, Michael J. Holdsworth, and Nicholas J. Provart
12. Identification of Seed Dormancy Mutants by Activation Tagging
Tiehan Zhao, Ying Zeng, and Allison R. Kermode
13. Isolation of MicroRNAs that Regulate Seed Dormancy and Germination
Arun Kumar M.B., Ruth C. Martin, and Hiro Nonogaki
14. Proteomics and Post-Translational Proteomics of Seed Dormancy and Germination
Loïc Rajjou, Maya Belghazi, Julie Catusse, Laurent Ogé, Erwann Arc, Béatrice Godin, Kamel Chibani, Sonia Ali-Rachidi, Boris Collet, Philippe Grappin, Marc Jullien, Karine Gallardo, Claudette Job, and Dominique Job
V. Epigenetic Changes Associated with Seed Dormancy
15. Chromatin Dynamics during Seed Dormancy
Yongxiu Liu, Regina Geyer, Vittoria Brambilla, Kazumi Nakabayashi, and Wim J.J. Soppe
16. Emerging Mass Spectrometry-Based Technologies for Analyses of Chromatin Changes: Analysis of Histones and Histone Modifications
Brinda Shah, Rachel L. Kozlowski, and Christoph H. Borchers
VI. Spectroscopic and Whole Tissue Analyses
17. In Vivo Nuclear Magnetic Resonance (NMR) Metabolite Profiling in Plant Seeds
Victor Terskikh and Allison R. Kermode
18. In Vivo 1H NMR Microimaging during Seed Imbibition, Germination, and Early Growth
Victor Terskikh, Kerstin Müller, Allison Kermode, and Gerhard Leubner-Metzger
19. Tissue Printing Methods for Localization of RNA and Proteins that Control Seed Dormancy and Germination
Wioletta E. Pluskota, Kent J. Bradford, and Hiro Nonogaki
VII. Analyses of Reactive Oxygen Species, Protein Damage and Repair
20. Analyses of Reactive Oxygen Species and Antioxidants in Relation to Seed Longevity and Germination
Christophe Bailly and Ilse Kranner
21. Protein Damage and Repair Controlling Seed Vigor and Longevity
Laurent Ogé, Caroline Broyart, Boris Collet, Béatrice Godin, Denis Jallet, Gildas Bourdais, Dominique Job, and Philippe Grappin
22. Nitric Oxide Methods in Seed Biology
Paul C. Bethke, Igor Libourel, Jan Vitecek, and Russell L. Jones
VIII. Bioinformatic Resources
23. Seed Bioinformatics
George W. Bassel, Michael J. Holdsworth, and Nicholas J. Provart
... weniger
Bibliographische Angaben
- 2011, 423 Seiten, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Allison R. Kermode
- Verlag: Humana Press
- ISBN-10: 1617792306
- ISBN-13: 9781617792304
- Erscheinungsdatum: 19.08.2011
Sprache:
Englisch
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