Signal Transduction Protocols
(Sprache: Englisch)
It is increasingly clear that signal transduction is a highly organized and integrated process. Divided into two convenient sections, Signal Transduction Protocols, Third Edition focuses on experimental approaches to better understand the complexity of...
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It is increasingly clear that signal transduction is a highly organized and integrated process. Divided into two convenient sections, Signal Transduction Protocols, Third Edition focuses on experimental approaches to better understand the complexity of signal transduction. Introductory chapters provide perspective on several of the challenges involved in signal transduction research and offer guidance on selecting the best approaches to various types of questions. The individual chapters provide detailed experimental protocols covering various topics, from the effects of ligand binding on receptor conformation and effector coupling, to moving inside the cell in order to capture the spatial and temporal characteristics of signaling events. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Signal Transduction Protocols serves as an ideal guide to scientists of all backgrounds and is a valuable resource for future progress in the field of signal transduction research.
Authoritative and accessible, Signal Transduction Protocols serves as an ideal guide to scientists of all backgrounds and is a valuable resource for future progress in the field of signal transduction research.
Klappentext zu „Signal Transduction Protocols “
It is increasingly clear that signal transduction is a highly organized and integrated process. Divided into two convenient sections, Signal Transduction Protocols, Third Edition focuses on experimental approaches to better understand the complexity of signal transduction. Introductory chapters provide perspective on several of the challenges involved in signal transduction research and offer guidance on selecting the best approaches to various types of questions. The individual chapters provide detailed experimental protocols covering various topics, from the effects of ligand binding on receptor conformation and effector coupling, to moving inside the cell in order to capture the spatial and temporal characteristics of signaling events. Written in the highly successful Methods in Molecular Biology¿ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Signal Transduction Protocols serves as an ideal guide to scientists of all backgrounds and is a valuable resource for future progress in the field of signal transduction research.
Inhaltsverzeichnis zu „Signal Transduction Protocols “
Part I. Overviews1. Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling
Louis M. Luttrell and Terry P. Kenakin
2. Imaging-based Approaches to Understanding G Protein-Coupled Receptor Signaling Complexes
Darlaine Pétrin and Terence E. Hébert
3. Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis
John K. Westwick and Jane E. Lamerdin
4. RGS-insensitive Ga Subunits: Probes of G¿ Subtype-selective Signaling and Physiological Functions of RGS Proteins
Kuljeet Kaur, Jason M. Kehrl, Raelene A. Charbeneau, and Richard R. Neubig
5. Bioinformatic Approaches to Metabolic Pathways Analysis
Stuart Maudsley, Wayne Chadwick, Liyun Wang, Yu Zhou, Bronwen Martin, and Sung Park
Part II. Receptor - Ligand Interactions
6. Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET
Jean-Pierre Vilardaga
7. Using BRET to Detect Ligand-Specific Conformational Changes in Preformed Signaling Complexes
Nicolas Audet and Graciela Piñeyro
Part III. Receptor - Receptor Interactions
8. Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High Density Lipoprotein Particles (rHDL)
Gisselle A. Vélez-Ruiz and Roger K. Sunahara
9. Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Hetero-Dimerization
Lamia Achour, Maud Kamal, Ralf Jockers, and Stefano Marullo
10. Cell-Surface Protein-Protein Interaction Analysis with Time-Resolved FRET and Snap-tag Technologies: Application to G Protein-Coupled Receptor Oligomerization
Laëtitia Comps-Agrar,
... mehr
Damien Maurel, Philippe Rondard, Jean-Philippe Pin, Trinquet Eric, and Laurent Prézeau
11. Analysis of GPCR/Ion Channel Interactions
Christophe Altier and Gerald W. Zamponi
Part IV. Receptor - Effector Coupling
12. Multicolor BiFC Analysis of G Protein ¿¿ Complex Formation and Localization
Thomas R. Hynes, Evan A. Yost, Stacy M. Yost, and Catherine H. Berlot
13. Real-Time BRET Assays to Measure G Protein/Effector Interactions
Darlaine Pétrin, Mélanie Robitaille, and Terence E. Hébert
14. Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP
Brock F. Binkowski, Frank Fan, and Keith V. Wood
15. Simultaneous Real Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters
Lianne B. Dale and Stephen S. G. Ferguson
Part V. Spatial Control of Signal Transduction
16. Using FRET-based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity
Charlene Depry and Jin Zhang
17. Genetically Encoded Fluorescent Reporters to Visualize Protein Kinase C Activation in Live Cells
Lisa L. Gallegos and Alexandra C. Newton
18. Visualizing Receptor Endocytosis and Trafficking
Ali Salahpour and Larry S. Barak
19. Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM
Guillermo A. Yudowski and Mark von Zastrow
20. Visualizing G Protein-Coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy
Sudha K. Shenoy
Part VI. Protein - Protein Interactions
21. Detection and Characterization of Receptor Interactions with PDZ Domains
Stefanie L. Ritter and Randy A. Hall
22. Tandem Affinity Purification and Identification of Heterotrimeric G-Protein Associated Proteins
Syed M. Ahmed, Avais M. Daulat, and Stéphane Angers
23. Study of G Protein-Coupled Receptor/ßarrestin Interactions within Endosomes Using FRAP
Benjamin Aguila, May Simaan, and Stéphane A. Laporte
24. Disrupting Protein Complexes Using Tat-tagged Peptide Mimics
Shupeng Li, Sheng Chen, Yu Tian Wang, and Fang Liu
25. Protein-fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic Studies of Protein-Protein Interactions in Living Cells
Stephen W. Michnick, Po Hien Ear, Christian Landry, Mohan K. Malleshaiah, and Vincent Messier
11. Analysis of GPCR/Ion Channel Interactions
Christophe Altier and Gerald W. Zamponi
Part IV. Receptor - Effector Coupling
12. Multicolor BiFC Analysis of G Protein ¿¿ Complex Formation and Localization
Thomas R. Hynes, Evan A. Yost, Stacy M. Yost, and Catherine H. Berlot
13. Real-Time BRET Assays to Measure G Protein/Effector Interactions
Darlaine Pétrin, Mélanie Robitaille, and Terence E. Hébert
14. Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP
Brock F. Binkowski, Frank Fan, and Keith V. Wood
15. Simultaneous Real Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters
Lianne B. Dale and Stephen S. G. Ferguson
Part V. Spatial Control of Signal Transduction
16. Using FRET-based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity
Charlene Depry and Jin Zhang
17. Genetically Encoded Fluorescent Reporters to Visualize Protein Kinase C Activation in Live Cells
Lisa L. Gallegos and Alexandra C. Newton
18. Visualizing Receptor Endocytosis and Trafficking
Ali Salahpour and Larry S. Barak
19. Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM
Guillermo A. Yudowski and Mark von Zastrow
20. Visualizing G Protein-Coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy
Sudha K. Shenoy
Part VI. Protein - Protein Interactions
21. Detection and Characterization of Receptor Interactions with PDZ Domains
Stefanie L. Ritter and Randy A. Hall
22. Tandem Affinity Purification and Identification of Heterotrimeric G-Protein Associated Proteins
Syed M. Ahmed, Avais M. Daulat, and Stéphane Angers
23. Study of G Protein-Coupled Receptor/ßarrestin Interactions within Endosomes Using FRAP
Benjamin Aguila, May Simaan, and Stéphane A. Laporte
24. Disrupting Protein Complexes Using Tat-tagged Peptide Mimics
Shupeng Li, Sheng Chen, Yu Tian Wang, and Fang Liu
25. Protein-fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic Studies of Protein-Protein Interactions in Living Cells
Stephen W. Michnick, Po Hien Ear, Christian Landry, Mohan K. Malleshaiah, and Vincent Messier
... weniger
Bibliographische Angaben
- 2011, 3rd ed., XIV, 430 Seiten, 10 farbige Abbildungen, 79 Schwarz-Weiß-Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Louis M. Luttrell, Stephen S. G. Ferguson
- Verlag: Springer-Verlag GmbH
- ISBN-10: 1617791598
- ISBN-13: 9781617791598
Sprache:
Englisch
Rezension zu „Signal Transduction Protocols “
From the reviews of the third edition:"This well-illustrated book on signal transduction protocols is a valuable resource for scientists interested in learning the intricate details of complex signaling events that take place within the cell ... . Although written for the scientists in the field of signal transduction research, it also will be useful for graduate students and other investigators in pharmacology interested in this area. ... Given the rapid advances in the field of signal transduction, this third edition is essential." (Omer Iqbal, Doody's Book Reviews, March, 2012)
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