Signal Transduction Protocols
(Sprache: Englisch)
In 1995, Signal Transduction Protocols, edited by David A. Kendall and Stephen J. Hill, was published in the Methods in Molecular Biology series. This second edition represents an update to that previous work with an emp- sis on new methodologies that have...
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In 1995, Signal Transduction Protocols, edited by David A. Kendall and Stephen J. Hill, was published in the Methods in Molecular Biology series. This second edition represents an update to that previous work with an emp- sis on new methodologies that have developed in the last few years. The goal, then and now, is to provide procedures written by experts with first-hand ex- rience in a detail that goes far beyond what is generally encountered in the "methods" section of most journals and thus actually permits a particular p- cedure to be replicated. In addition, we have had as a secondary goal the id- tification of protocols for the assay of general classes of signal transduction components that, ideally, can be adapted to the assay of any member of that class. The ability to do this has resulted in large part from the use of affini- based assays, the ease with which specific proteins can be specifically tagged, and an explosion in the availability of highly specific antibodies from comm- cial sources, especially antibodies raised against signaling proteins of human origin. The number of available approaches is, fortunately for those working in signaling research, far too great to fit within the confines of this volume, so hard choices as to what to include had to be made.
Inhaltsverzeichnis zu „Signal Transduction Protocols “
Making Protein ImmunoprecipitatesElaine A. Elion and Yunmei Wang
Signal Transduction Inhibitors in Cellular Function
Maofu Fu, Chenguang Wang, Xueping Zhang, and Richard G. Pestell
Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets
Yukihito Kabuyama, Kirsi K. Polvinen, Katheryn A. Resing, and Natalie G. Ahn
A High-Throughput Mammalian Cell-Based Transient Transfection Assay
Daniel J. Noonan, Kenneth Henry, and Michelle L. Twaroski
Determining Protein Half-Lives
Pengbo Zhou
Assaying Protein Kinase Activity
Jan Brábek and Steven K. Hanks
Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Based Approach
Francesca Zappacosta, Michael J. Huddleston, and Roland S. Annan
Studies of Calmodulin-Dependent Regulation
Paul C. Brandt and Thomas C. Vanaman
Measurement of Protein-DNA Interactions In Vivo by Chromatin Immunoprecipitation
Hogune Im, Jeffrey A. Grass, Kirby D. Johnson, Meghan E. Boyer, Jing Wu, and Emery H. Bresnick
Characterization of Protein-DNA Association In Vivo by Chromatin Immunoprecipitation
Laurent Kuras
Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins
Douglas A. Andres
Nucleocytoplasmic Glycosylation, O-GlcNAc: Identification and Site Mapping
Natasha Elizabeth Zachara, Win Den Cheung, and Gerald Warren Hart
Techniques in Protein Methylation
Jaeho Lee, Donghang Cheng, and Mark T. Bedford
Assaying Lipid Phosphate Phosphatase Activities
Gil-Soo Han and George M. Carman
Assaying Phosphoinositide Phosphatases
Gregory S. Taylor and Jack E. Dixon
Assaying Phospholipase A2 Activity
Christina C. Leslie and Michael H. Gelb
Measurement and Immunofluorescence of Cellular Phosphoinositides
Hiroko Hama,Javad Torabinejad, Glenn D. Prestwich, and Daryll B. DeWald
Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer
Sandrine Evellin, Marco Mongillo, Anna Terrin, Valentina Lissandron, and Manuela Zaccolo
In
... mehr
Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET
Chitra Subramanian, Yao Xu, Carl Hirschie Johnson, and Albrecht G. von Arnim
Revealing Protein Dynamics by Photobleaching Techniques
Frank van Drogen and Matthias Peter
Assaying Cytochrome c Translocation During Apoptosis
Nigel J. Waterhouse, Rohan Steel, Ruth Kluck, and Joseph A. Trapani
Index
Chitra Subramanian, Yao Xu, Carl Hirschie Johnson, and Albrecht G. von Arnim
Revealing Protein Dynamics by Photobleaching Techniques
Frank van Drogen and Matthias Peter
Assaying Cytochrome c Translocation During Apoptosis
Nigel J. Waterhouse, Rohan Steel, Ruth Kluck, and Joseph A. Trapani
Index
... weniger
Bibliographische Angaben
- 2010, 2. Aufl., 327 Seiten, mit Abbildungen, Maße: 22,9 cm, Kartoniert (TB), Englisch
- Herausgegeben von Dickson, Robert C.; Mendenhall, Michael D.
- Verlag: Springer
- ISBN-10: 1617374482
- ISBN-13: 9781617374487
Sprache:
Englisch
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