Synthetic Methods for Biologically Active Molecules

Preface
 
DEVELOPMENT OF SUSTAINABLE BIOCATALYTIC REDUCTION PROCESSES FOR ORGANIC CHEMISTS
Introduction
Biocatalytic Reductions of C=O Double Bonds
Biocatalytic Reductions of C=C Double Bonds
Biocatalytic Reductions of Imines to Amines
Biocatalytic Reductions of Nitriles to Amines
Biocatalytic Deoxygenation Reactions
Emerging Reductive Biocatalytic Reactions
Reaction Engineering for Biocatalytic Reduction Processes
Summary and Outlook
 
REDUCTASES: FROM NATURAL DIVERSITY TO ESTABLISHED BIOCATALYSIS AND TO EMERGING ENZYMATIC ACTIVITIES
Reductases: Natural Occurrence and Context for Biocatalysis
Emerging Cases of Reductases in Biocatalysis
Concluding Remarks
 
SYNTHETIC STRATEGIES BASED ON C=C BIOREDUCTIONS FOR THE PREPARATION OF BIOLOGICALLY ACTIVE MOLECULES
Introduction
Bioreduction of Alpha,Beta-Unsaturated Carbonyl Compounds
Bioreduction of Nitroolefins
Bioreduction of Alpha,Beta-Unsaturated Carboxylic Acids and Derivatives
Bioreduction of Alpha,Beta-Unsaturated Nitriles
Concluding Remarks
 
SYNTHETIC STRATEGIES BASED ON C=O BIOREDUCTIONS FOR THE PREPARATION OF BIOLOGICALLY ACTIVE MOLECULES
Introduction
Synthesis of Biologically Active Compounds through C=O Bioreduction
Other Strategies to Construct Biologically Active Compounds
Summary and Outlook
 
PROTEIN ENGINEERING: DEVELOPMENT OF NOVEL ENZYMES FOR THE IMPROVED REDUCTION OF C=C DOUBLE BONDS
Introduction
The Protein Engineering Process and Employed Mutagenesis Methods
Examples of Rational Design of Old Yellow Enzymes
Evolving Old Yellow Enzymes (OYEs)
Conclusions and Perspectives
 
PROTEIN ENGINEERING: DEVELOPMENT OF NOVEL ENZYMES FOR THE IMPROVED REDUCTION OF C=O DOUBLE BONDS
Introduction
Detailed Characterization of PAR
Detailed Characterization of LSADH
Engineering of PAR for Increasing Activity in 2-Propanol/Water Medium
Application of Whole-Cell Biocatalysts Possessing Mutant PARs and LSADH
Engineering of Beta-Keto Ester Reductase

Elisabetta Brenna received her laurea (1989) and PhD (1993) in chemistry from the University of Milan. In 1996, she became Assistant Professor at Politecnico di Milano, where she is now Associate Professor. Her main scientific interests are the enzyme-mediated synthesis of the single enantiomers of chiral biologically active compounds, and the use of the SNIF NMR technique for tracing back the synthetic history of active pharmaceutical ingredients and synthetic flavourings. She is author of more than 120 publications in international journals.