The ESCRT Complexes
Methods and Protocols
(Sprache: Englisch)
This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S....
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This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering biochemical approaches presenting strategies for production and characterization of recombinant ESCRT proteins, or of specific ESCRT protein domains, as well as genetic and proteomic experimental approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The ESCRT Complexes: Methods and Protocols serves as a compact guide for researchers interested in establishing an integrated approach to investigate the ESCRT machinery functions in cell biology.
Inhaltsverzeichnis zu „The ESCRT Complexes “
1. Analysis of the Archaeal ESCRT ApparatusRachel Y. Samson, Iain G. Duggin, and Stephen D. Bell
2. Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster
Marco Gualtieri and Thomas Vaccari
3. Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc
Tamas Matusek, Pascal Thérond, and Maximilian Fürthauer
4. Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative-Light Electron Microscopy
Céline Largeau, Emmanuel Culetto, and Renaud Legouis
5. Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells
Binod Kumar, Mohanan Valiya Veettil, Arunava Roy, and Bala Chandran
6. Immuno-Localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy
Keisuke Tabata, Atsuki Nara, Hiroko Omori, and Eiji Morita
7. Single Cell Fluorescence Ratio Image Analysis for Studying ESCRT Function in Receptor Trafficking Jalal M. Kazan, Gergely L. Lukacs, Pirjo M. Apaja, and Arnim Pause
8. Genetic and Biochemical Analyses of Yeast ESCRT
... mehr
Sudeep Banjade, Shaogeng Tang, and Scott D. Emr
9. Live Imaging of ESCRT Proteins in Microfluidically-Isolated Hippocampal Axons
Veronica Birdsall, Jose C. Martinez, Lisa Randolph, Ulrich Hengst, and Clarissa L. Waites
10. Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High Resolution Imaging Techniques SIM and Cryo-SXT
Shai Adar-Levor, Inna Goliand, Michael Elbaum, and Natalie Elia
11. Three Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software
Romain Le Bars, Michele W. Bianchi, and Christophe Lefebvre
12. Transient Expression of ESCRT-Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts
Marie-Kristin Nagel, Karin Vogel, and Erika Isono
13. Crystallization and Biophysical Approaches for Studying the Interactions between the Vps4-MIT Domain and ESCRT-III Proteins
Takayuki Obita, Rieko Kojima, and Mineyuki Mizuguchi
14. Biochemical Approaches to Studying C. elegans ESCRT Function In Vitro
Samuel Block, Amber L. Schuh, and Anjon Audhya
15. Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays
Luisa Capalbo, Ioanna Mela, Maria Alba Abad, A. Arockia Jeyaprakash, J. Michael Edwardson, and Pier Paolo D'Avino
16. Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting
Francheska Son, Katharine Umphred-Wilson, Jae-Hyuck Shim, and Stanley Adoro
17. Purification of Plant ESCRT Proteins for Polyclonal Antibody Production Julio Paez-Valencia and Marisa S. Otegui
18. Genetic and Cytological Methods to Study ESCRT Cell Cycle Function in Fission Yeast
Imane M. Rezig, Shaun K. Bremner, Musab S. Bhutta, Ian P. Salt, Gwyn W. Gould, and Christopher J. McInerny
19. ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis
Carl Haag, Thomas Klein, and Michael Feldbrügge
20. Genetic Suppressor Screen Using an Inducible FREE1-RNAi Line to Detect ESCRT Genetic Interactors in Arabidopsis thaliana
Qiong Zhao, Ying Zhu, Wenhan Cao, Jinbo Shen, Yong Cui, Shuxian Huang, and Liwen Jiang
21. Screening of Interactions with the ESCRT Machinery by a Gaussia princeps Split Luciferase-Based Complementation Assay
Rina Barouch-Bentov, Yves Jacob, and Shirit Einav
22. RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells
Katherine Bowers
Sudeep Banjade, Shaogeng Tang, and Scott D. Emr
9. Live Imaging of ESCRT Proteins in Microfluidically-Isolated Hippocampal Axons
Veronica Birdsall, Jose C. Martinez, Lisa Randolph, Ulrich Hengst, and Clarissa L. Waites
10. Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High Resolution Imaging Techniques SIM and Cryo-SXT
Shai Adar-Levor, Inna Goliand, Michael Elbaum, and Natalie Elia
11. Three Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software
Romain Le Bars, Michele W. Bianchi, and Christophe Lefebvre
12. Transient Expression of ESCRT-Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts
Marie-Kristin Nagel, Karin Vogel, and Erika Isono
13. Crystallization and Biophysical Approaches for Studying the Interactions between the Vps4-MIT Domain and ESCRT-III Proteins
Takayuki Obita, Rieko Kojima, and Mineyuki Mizuguchi
14. Biochemical Approaches to Studying C. elegans ESCRT Function In Vitro
Samuel Block, Amber L. Schuh, and Anjon Audhya
15. Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays
Luisa Capalbo, Ioanna Mela, Maria Alba Abad, A. Arockia Jeyaprakash, J. Michael Edwardson, and Pier Paolo D'Avino
16. Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting
Francheska Son, Katharine Umphred-Wilson, Jae-Hyuck Shim, and Stanley Adoro
17. Purification of Plant ESCRT Proteins for Polyclonal Antibody Production Julio Paez-Valencia and Marisa S. Otegui
18. Genetic and Cytological Methods to Study ESCRT Cell Cycle Function in Fission Yeast
Imane M. Rezig, Shaun K. Bremner, Musab S. Bhutta, Ian P. Salt, Gwyn W. Gould, and Christopher J. McInerny
19. ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis
Carl Haag, Thomas Klein, and Michael Feldbrügge
20. Genetic Suppressor Screen Using an Inducible FREE1-RNAi Line to Detect ESCRT Genetic Interactors in Arabidopsis thaliana
Qiong Zhao, Ying Zhu, Wenhan Cao, Jinbo Shen, Yong Cui, Shuxian Huang, and Liwen Jiang
21. Screening of Interactions with the ESCRT Machinery by a Gaussia princeps Split Luciferase-Based Complementation Assay
Rina Barouch-Bentov, Yves Jacob, and Shirit Einav
22. RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells
Katherine Bowers
... weniger
Bibliographische Angaben
- 2019, 1st ed. 2019, XIV, 322 Seiten, 43 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Emmanuel Culetto, Renaud Legouis
- Verlag: Springer, Berlin
- ISBN-10: 1493994913
- ISBN-13: 9781493994915
- Erscheinungsdatum: 02.07.2019
Sprache:
Englisch
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