Tissue Morphogenesis

PART I IMAGING MORPHOGENESIS

Chapter 1. Probing Regional Mechanical Properties of Embryonic Tissue Using Microindentation and Optical Coherence Tomography

Benjamin A. Filas, Gang Xu, and Larry A. Taber

Chapter 2. Hemodynamic Flow Visualization of Early Embryonic Great Vessels using mPIV

Selda Goktas, Chia-Yuan Chen, William J. Kowalski, and Kerem Pekkan

Chapter 3. Using Correlative Light and Electron Microscopy to Study Zebrafish Vascular Morphogenesis

Jacky G. Goetz, Fabien Monduc, Yannick Schwab, and Julien Vermot

Chapter 4. Micro/Nano-Computed Tomography Technology for Quantitative Dynamic, Multi-scale Imaging of Morphogenesis

Chelsea L. Gregg, Andrew K. Recknagel, and Jonathan T. Butcher

Chapter 5. Imaging the Dorsal-Ventral Axis of Live and Fixed Drosophila Melanogaster Embryos

Sophia N. Carrell and Gregory T. Reeves

Chapter 6. Light-Sheet-Based Imaging and Analysis of Early Embryogenesis in the Fruit Fly

Khaled Khairy, William Lemon, Fernando Amat, and Philipp J. Keller

Chapter 7. Quantitative Image Analysis of Cell Behavior and Molecular Dynamics During Tissue Morphogenesis

Chun Yin Bosco Leung and Rodrigo Fernandez-Gonzalez

Chapter 8. A Multiplex Fluorescent in situ Hybridization Protocol for Clonal Analysis of Drosophila Oogenesis

Lily S. Cheung and Stanislav Shvartsman

Chapter 9. Active Cell and ECM Movements During Development

Anastasiia Aleksandrova, Brenda J. Rongish, Charles D. Little, and Andras Czirok

PART II CULTURE MODELS OF MORPHOGENESIS

Chapter 10. 3D Culture Assays of Murine Mammary Branching Morphogenesis and Epithelial Invasion

Kim-Vy Nguyen-Ngoc, Eliah R. Shamir, Robert J. Huebner, Jennifer N. Beck, Kevin J. Cheung, and Andrew J. Ewald

Chapter 11. Culture of Mouse Embryonic Foregut Explants

Felicia Chen and Wellington V. Cardoso

Chapter 12. Investigating Human Vascular Tube Morphogenesis and maturation using endothelial cell-pericyte co-cultures and a