Xenopus Protocols
Post-Genomic Approaches
(Sprache: Englisch)
During the 20th century Xenopus has flourished as an accessible experimental model system for studying vertebrate biology. The second edition of Xenopus Protocols:Post-Genomic Approaches expands upon the previous edition with novel approaches inspired by...
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During the 20th century Xenopus has flourished as an accessible experimental model system for studying vertebrate biology. The second edition of Xenopus Protocols:Post-Genomic Approaches expands upon the previous edition with novel approaches inspired by Xenopus tropicalis and the sequencing of its genome. Xenopus tropicalis genomics and tractable genetics combined with the unique experimental advantages of Xenopus laevis and their distinctive phylogenetic position make them unique model systems for vertebrate embryonic development in the 21st century. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Xenopus Protocols: Post-Genomic Approaches, Second Edition provides a toolkit that will enable every laboratory to maximize the power of this extraordinary experimental system in the post-genomic age.
Authoritative and practical, Xenopus Protocols: Post-Genomic Approaches, Second Edition provides a toolkit that will enable every laboratory to maximize the power of this extraordinary experimental system in the post-genomic age.
Klappentext zu „Xenopus Protocols “
During the 20th century Xenopus has flourished as an accessible experimental model system for studying vertebrate biology. The second edition of Xenopus Protocols:Post-Genomic Approaches expands upon the previous edition with novel approaches inspired by Xenopus tropicalis and the sequencing of its genome. Xenopus tropicalis genomics and tractable genetics combined with the unique experimental advantages of Xenopus laevis and their distinctive phylogenetic position make them unique model systems for vertebrate embryonic development in the 21st century. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Xenopus Protocols: Post-Genomic Approaches, Second Edition provides a toolkit that will enable every laboratory to maximize the power of this extraordinary experimental system in the post-genomic age.
Inhaltsverzeichnis zu „Xenopus Protocols “
Part I: Xenopus Tropicalis 1. Xenopus tropicalis as a Model Organism for Genetics and Genomics: Past, Present and Future
Robert M. Grainger
2. Husbandry of Xenopus tropicalis
Alan Jafkins, Anita Abu-Daya, Anna Noble, Lyle Zimmerman, and Matthew Guille
3. Generating Diploid Embryos from Xenopus tropicalis
Florencia del Viso and Mustafa Khokha
4. Navigating the Xenopus tropicalis Genome
Ira L. Blitz
Part II: Genetics in Xenopus
5. Genetic Analysis of Xenopus tropicalis
Timothy J. Geach, Derek L. Stemple, and Lyle B. Zimmerman
6. Forward Genetic Screens in Xenopus using Transposon-mediated Insertional Mutagenesis
Donald A. Yergeau, Clair M. Kelley, Haiqing Zhu, Emin Kuliyev and Paul E. Mead
7. Targeted Gene Disruption with Engineered Zinc-finger Nucleases (ZFNs)
John J. Young and Richard M. Harland
8. Reverse Genetic Studies using Antisense Morpholino Oligonucleotides
Yanan Zhao, Shoko Ishibashi and Enrique Amaya
9. Chemical Genetics and Drug Discovery in Xenopus
Matthew L. Tomlinson, Adam Hendry, and Grant N. Wheeler
10. Maternal mRNA Knock-down Studies: Antisense Experiments using the Host-transfer Technique in Xenopus laevis and Xenopus tropicalis
David J. Olson, Alissa M. Hulstrand and Douglas W. Houston
Part II: Xenopus Trangenesis
11. Generating Transgenic Frog Embryos by Restriction Enzyme Mediated Integration (REMI)
Shoko Ishibashi, Kristen L. Kroll and Enrique Amaya
12. A Simple Method of Transgenesis using I-SceI meganuclease in Xenopus
Shoko Ishibashi, Nick R. Love and Enrique Amaya
13. Using FC31 Integrase to Mediate Insertion of DNA in Xenopus Embryos
You E. Li, Bryan G. Allen and Daniel L. Weeks
14. Xenopus Transgenics: Methods using Transposons
Clair M. Kelley,
... mehr
Donald A. Yergeau, Haiqing Zhu, Emin Kuliyev and Paul E. Mead
15. Comparative Genomics-based Identification and Analysis of Cis-regulatory Elements
Hajime Ogino, Haruki Ochi, Chihiro Uchiyama, Sarah Louie, and Robert M. Grainger
16. Tet-On Binary Systems for Tissue-specific and
Inducible Transgene Expression
Daniel R. Buchholz
Part III: Gene Expression: From Gene to Transcript to Protein
17. Chromatin Immunoprecipitation Analysis of Xenopus Embryos
Robert C. Akkers, Ulrike G. Jacobi and Gert Jan C. Veenstra
18. Transcriptomics Using Next Generation Sequencing Technologies
Dasfne Lee-Liu, Leonardo I. Almonacid, Fernando Faune, Francisco Melo, Juan Larrain
19. Databases of Gene Expression in Xenopus Development
Michael J. Gilchrist, Nicolas Pollet
20. Investigating Alternative RNA Splicing in Xenopus
Agnès Mereau and Serge Hardy
21. Immunoisolation of Protein Complexes from Xenopus
Frank L. Conlon, Yana Miteva, Erin Kaltenbrun, Lauren Waldron, Todd M. Greco, Ileana M. Cristea
22. Complementary Proteomic Analysis of Protein Complexes
Todd M. Greco, Yana Miteva, Frank L. Conlon, Ileana M. Cristea
Part IV: Imaging Xenopus Development
23. Antibody Development and use in Chromogenic and Fluorescent Immunostaining
Eamon Dubaissi, Niki Panagiotaki, Nancy Papalopulu, and Peter D. Vize
24. Multiple fluorescent in situ mRNA Hybridization (FISH) on Whole Mounts and Sections
Robert Lea, Boyan Bonev, Eamon Dubaissi, Peter D. Vize and Nancy Papalopulu
25. Methods to Analyze microRNA Expression and Function During Xenopus Development
Boyan Bonev and Nancy Papalopulu
26. A Bromodeoxyuridine (BrdU) Based Protocol for Characterizing Proliferating Progenitors in Xenopus Embryos
Helene Auger, Raphael Thuret, Warif El Yakoubi, and Nancy Papalopulu
27. Microscopy Tools for Quantifying Developmental Dynamics in Xenopus Embryos
Sagar D. Joshi, Hye Young Kim, Lance A. Davidson
Part V: Novel Approaches in Xenopus
28. Mathematical Modelling of Gene RegulatoryNetworks in Xenopus Development
Yasushi Saka
29. Stem-cell-like Embryonic Explants to Study Cardiac Development
Boni A. Afouda
30. Studying Regeneration in Xenopus
Caroline W. Beck
31. On-line Resources for Xenopus
Jeff Bowes
Boyan Bonev and Nancy Papalopulu
15. Comparative Genomics-based Identification and Analysis of Cis-regulatory Elements
Hajime Ogino, Haruki Ochi, Chihiro Uchiyama, Sarah Louie, and Robert M. Grainger
16. Tet-On Binary Systems for Tissue-specific and
Inducible Transgene Expression
Daniel R. Buchholz
Part III: Gene Expression: From Gene to Transcript to Protein
17. Chromatin Immunoprecipitation Analysis of Xenopus Embryos
Robert C. Akkers, Ulrike G. Jacobi and Gert Jan C. Veenstra
18. Transcriptomics Using Next Generation Sequencing Technologies
Dasfne Lee-Liu, Leonardo I. Almonacid, Fernando Faune, Francisco Melo, Juan Larrain
19. Databases of Gene Expression in Xenopus Development
Michael J. Gilchrist, Nicolas Pollet
20. Investigating Alternative RNA Splicing in Xenopus
Agnès Mereau and Serge Hardy
21. Immunoisolation of Protein Complexes from Xenopus
Frank L. Conlon, Yana Miteva, Erin Kaltenbrun, Lauren Waldron, Todd M. Greco, Ileana M. Cristea
22. Complementary Proteomic Analysis of Protein Complexes
Todd M. Greco, Yana Miteva, Frank L. Conlon, Ileana M. Cristea
Part IV: Imaging Xenopus Development
23. Antibody Development and use in Chromogenic and Fluorescent Immunostaining
Eamon Dubaissi, Niki Panagiotaki, Nancy Papalopulu, and Peter D. Vize
24. Multiple fluorescent in situ mRNA Hybridization (FISH) on Whole Mounts and Sections
Robert Lea, Boyan Bonev, Eamon Dubaissi, Peter D. Vize and Nancy Papalopulu
25. Methods to Analyze microRNA Expression and Function During Xenopus Development
Boyan Bonev and Nancy Papalopulu
26. A Bromodeoxyuridine (BrdU) Based Protocol for Characterizing Proliferating Progenitors in Xenopus Embryos
Helene Auger, Raphael Thuret, Warif El Yakoubi, and Nancy Papalopulu
27. Microscopy Tools for Quantifying Developmental Dynamics in Xenopus Embryos
Sagar D. Joshi, Hye Young Kim, Lance A. Davidson
Part V: Novel Approaches in Xenopus
28. Mathematical Modelling of Gene RegulatoryNetworks in Xenopus Development
Yasushi Saka
29. Stem-cell-like Embryonic Explants to Study Cardiac Development
Boni A. Afouda
30. Studying Regeneration in Xenopus
Caroline W. Beck
31. On-line Resources for Xenopus
Jeff Bowes
Boyan Bonev and Nancy Papalopulu
... weniger
Bibliographische Angaben
- 2012, 2. Aufl., XIII, 568 Seiten, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Stefan Hoppler, Peter D Vize
- Verlag: Springer, Berlin
- ISBN-10: 1617799912
- ISBN-13: 9781617799914
- Erscheinungsdatum: 06.09.2012
Sprache:
Englisch
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