Alternative pre-mRNA Splicing (ePub)

Companion Website: www.wiley-vch.de/home/splicing INTRODUCTION Splicing in the RNA World (Emanuele Buratti, Maurizio Romano and Francisco E. Baralle) RNPs, small RNAs, miRNAs (Michaela Beitzinger and Gunter Meister) RNA Elements Involved in Splicing (William F. Mueller and Klemens J. Hertel) A Structural Biology Perspective of Proteins Involved in Splicing Regulation (Antoine Cléry and Frederic Allain) The Spliceosome in Constitutive Splicing (Patrizia Fabrizio and Reinhard Lührmann) The Use of Saccharomyces cerevisiae to Study the Mechanism of pre-mRNA Splicing (Brian C. Rymond) Challenges in Plant Alternative Splicing (Andrea Barta, Yamile Marquez and John WS Brown) Alternative Splice Site Selection (Miguel B. Coelho and Christopher WJ Smith) Integration of Splicing with Nuclear and Cellular Events (Aparna K. Sapra and Carla Neugebauer) Splicing and Disease (Emanuele Buratti and Francisco E. Baralle) From Bedside to Bench: How to Analyze a Splicing Mutation (Marco Baralle and Diana Baralle) Analysis of Splicing Problems (Stefan Stamm) BASIC METHODS Ultracentrifugation in the Analysis and Purification by of Spliceosomes Assembled in Vitro (Klaus Hartmuth, Maria A. van Santen and Reinhard Lührmann) Chemical Synthesis of RNA (Claudia Höbartner) RNA Interference (siRNA, ShRNA) (Daphne S. Cabianca and Davide Gabellini) Expression and Purification of Splicing Proteins (James Stévenin and Cyril F. Bourgeois) Detection of RNA-Protein Complexes By Electrophoretic Mobility Shift Assay (Manli Shen and Michael G. Fried) Functional Analysis of large Exonic Sequences through Iterative in Vivo Selection (Natalia N. Singh, Joonbae Seo and Ravindra N. Singh) Identification of Splicing cis-Elements through an Ultra-Refined Antisense Microwalk (Ravindra N. Singh and Natalia N. Singh) Genomic SELEX to Identify RNA Targets of Plant RNA Binding Proteins (Olga Bannikova and Andrea Barta) DETECTION OF SPLICING EVENTS Quantification of Alternative Splice Variants (Miriam LLorian and

Stefan Stamm is Associate Professor in the Department for Molecular and Cellular Biochemistry at the University of Kentucky. He studied Biochemistry in Hannover (Germany) and did the practical work for his PhD as well as postdoctoral work at the Cold Spring Harbor Laboratory, NY. His research focuses on mechanisms and regulation of alternative splicing, with
the aim to apply the fi ndings to the Prader-Willi Syndrome and Spinal Muscular Atrophy.

Chris Smith is Professor of RNA Molecular Biology in the Department of Biochemistry at the University of Cambridge. His PhD research in Biochemistry was carried out at the University of London, followed by postdoctoral research at Harvard Medical School. His research interests encompass the mechanisms, regulation and function of alternative splicing.

Reinhard Lührmann is Director at the Max Planck Institute for Biophysical Chemistry in Göttingen where he is head of the Department of Cellular Biochemistry. He is an Honorary Professor at the University of Göttingen. He has studied chemistry at the University of Münster where he also received his PhD. He worked as a postdoctoral fellow at the Max Planck Institute
for Molecular Genetics in Berlin. His research focuses on the structure and mechanism of the spliceosome.

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