Engineering the Genetic Code (PDF)
Expanding the Amino Acid Repertoire for the Design of Novel Proteins
(Sprache: Englisch)
The ability to introduce non-canonical amino acids in vivo has greatly expanded the repertoire of accessible proteins for basic research and biotechnological application.
Here, the different methods and strategies to incorporate new or modified amino acids...
Here, the different methods and strategies to incorporate new or modified amino acids...
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The ability to introduce non-canonical amino acids in vivo has greatly expanded the repertoire of accessible proteins for basic research and biotechnological application.
Here, the different methods and strategies to incorporate new or modified amino acids are explained in detail, including a lot of practical advice for first-time users of this powerful technique.
Novel applications in protein biochemistry, genomics, biotechnology and biomedicine made possible by the expansion of the genetic code are discussed and numerous examples are given.
Essential reading for all molecular life scientists who want to stay ahead in their research.
Here, the different methods and strategies to incorporate new or modified amino acids are explained in detail, including a lot of practical advice for first-time users of this powerful technique.
Novel applications in protein biochemistry, genomics, biotechnology and biomedicine made possible by the expansion of the genetic code are discussed and numerous examples are given.
Essential reading for all molecular life scientists who want to stay ahead in their research.
Inhaltsverzeichnis zu „Engineering the Genetic Code (PDF)“
INTRODUCTION Classical Approaches for Protein Modification Protein Total Synthesis Basic Definitions and Taxonomy HISTORY OF AN EXPANDED AMINO ACID REPERTOIRE Cell-free Synthesis of Proteins Noncanonical Amino Acids as Tools for Studying Metabolism Genetic Code Engineering BASIC FEATURES OF THE CELLULAR TRANSLATION APPARATUS Ribosome-mediated Protein Synthesis tRNAs and tRNA Synthetases Translational Proofreading Ribosomal Decoding, Codon Bias and Translational Fidelity Context-dependent Recoding Post-translational Modifications GENETIC CODE ORGANISATION AND PROTEIN STRUCTURE The Universal Genetic Code Natural Variations in Codon Assignment Codon Reassignment and Codon Ambiguity REPROGRAMMING THE CELLULAR TRANSLATION MACHINERY Enzyme Specificity and Code Interpretation Reassigning Coding and Non-Coding Units In vitro Chemical and Enzymatic tRNA Aminoacylation Novel Codon-Anticodon Base Pairs Stop Codon Takover Suppression-Based Methods Future Approaches IMPLICATIONS AND INSIGHTS Evolution of the Amino Acid Repertoire Artificial Genetic Systems De novo Design of Organisms Chances and Risks APPLICATIONS FOR REPROGRAMMED CELLULAR TRANSLATION Genetically Encoded Protein Surface Diversity Applications in Structural Biology Atomic Mutations Protein Folding, Stability and Design Tailoring Protein Spectroscopy Protein-based Sensors Applications in Biomedicine Noncanonical Amino Acids with Neurological Activity
Autoren-Porträt von Nediljko Budisa
Nediljko Budisa is a group leader at the Max-Planck-Institute of Biochemistry in Martinsried near Munich (Germany). He studied Chemistry and Biology at the University of Zagreb (Croatia) before joining the group of Nobel Prize Winner Robert Huber at Martinsried to obtain his PhD degree. During postdoctoral work with R. Huber and L. Moroder he led an independent research team in protein engineering. In 2004, Dr. Budisa received the BioFuture Award of the German Federal Ministry for Research and Education.
Bibliographische Angaben
- Autor: Nediljko Budisa
- 2006, 1. Auflage, 296 Seiten, Englisch
- Verlag: Wiley-VCH
- ISBN-10: 3527607099
- ISBN-13: 9783527607099
- Erscheinungsdatum: 12.05.2006
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- Größe: 5.37 MB
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