Advanced Bacterial Genetics: Use of Transposons and Phage for Genomic Engineering

Section I. Strain Collections and Genetic Nomenclature. Chapter 1. Strain Collections and Genetic Nomenclature. Section II. Transposons. Chapter 2: Use of Antibiotic Resistant Transposons for Mutagenesis. Chapter 3: In vivo Mutagenesis Using EZ-Tn5TM. Chapter 4: Identification of essential genes in bacteria. Chapter 5: Isolation and use of linked transposons. Chapter 6: Localized mutagenesis. Chapter 7: Generation of deletions and duplications using transposons as portable regions of homology with emphasis on Mud and Tn10 transposons. Chapter 8: Target-Directed Proteolysis in vivo. Chapter 9: Sets of transposon generated sequence-tagged mutants for structure-function analysis and engineering. Chapter 10: Using Genomic Microarrays to Study Insertional/Transposon Mutant Libraries. Chapter 11: Screening Transposon Mutant Libraries Using Full-Genome Oligonucleotide Microarrays. Chapter 12: Creating recombination-activated genes and sequence-defined mutant libraries using transposons Chapter 13: Use of transposons to study genetic regulation fusions. Chapter 14: Genomic screening for regulatory genes using the T-POP transposon. Section III. Phage. Chapter 15: Recombineering: in vivo genetic engineering in E. coli, S. enterica and beyond. Chapter 16: U-Red Genetic Engineering in Salmonella enterica serovar Typhimurium. Chapter 17: Integrase. Chapter 18: Challenge phage. Chapter 19: MudP22. Chapter 20: Phage metagenomics.