Animal Cell Biotechnology

Animal Cell Biotechnology - Methods and ProtocolsEditor: Ralf PörtnerContentsPrefaceContributorsPart I. Basic techniques for establishing production cell lines1 Transduction TechnologiesDavid A. Fluri and Martin Fussenegger2 Cells for gene therapy and vector productionC. Delenda, M. Chillon, A.-M. Douar and Otto-Wilhelm Merten3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody FragmentsAlexandra Dorn-Beineke, Stefanie Nittka and Michael Neumaier4 DNA Fingerprinting and Characterisation of Animal Cell LinesGlyn N. Stacey, Ed Byrne and J. Ross HawkinsPart II. Basic cultivation techniques5 Microcarrier Cell culture TechnologyGerald Blüml6 Cell EncapsulationHolger Hübner7 Tools for High-Throughput Medium and Process OptimisationMartin Jordan and Nigel JenkinsPart III. Cell characterisation and analysis8 Cell counting and viability measurementsMichael Butler and Maureen Spearman9 Monitoring of Growth, Physiology and Productivity of animal cells by Flow CytometrySilvia Carroll, Mariam Nasiri and Mohamed Al-Rubeai10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive BioreactorsAndré A. Neves and Kevin M. Brindle11 Methods for off-line analysis of nutrients and products in mammalian cell cultureHeino Büntemeyer12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product FormationDerek Adams, Rashmi Korke, and Wei-Shou Hu13 Measurement of apoptosis in cell cultureAdiba Ishaque1 and Mohamed Al-Rubeai14 Metabolic Flux Estimation in Mammalian Cell CulturesChetan T. Goudar, Richard Biener, JamesM. Piret and Konstantin B. KonstantinovPart IV. Special cultivation techniques15 Disposable bioreactors for inoculum production and protein expressionRegine Eibl and Dieter Eibl16 Hollow Fibre Cell CultureJohn M. Davis17 Cultivation of mammalian cells in fixed bed reactorsRalf Pörtner and Oscar B.J. Platas Barradas18 Configuration of BioreactorsDirk E. Martens and Evert Jan van den