Molecular Biotechnology
Principles and Applications of Recombinant DNA
(Sprache: Englisch)
Molecular Biotechnology
Molecular Biotechnology
Principles and Applications of Recombinant DNA
SIXTH EDITION
An authoritative introduction to the fast-changing world of molecular biotechnology
In continuous...
Molecular Biotechnology
Principles and Applications of Recombinant DNA
SIXTH EDITION
An authoritative introduction to the fast-changing world of molecular biotechnology
In continuous...
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Klappentext zu „Molecular Biotechnology “
Molecular BiotechnologyMolecular Biotechnology
Principles and Applications of Recombinant DNA
SIXTH EDITION
An authoritative introduction to the fast-changing world of molecular biotechnology
In continuous publication since 1994 and now in its sixth edition, Molecular Biotechnology: Principles and Applications of Recombinant DNA has been effective in introducing this complex field to students for more than 25 years. This textbook covers essentially every aspect of the field of molecular biotechnology, which is constantly changing and adapting in light of new advances.
This edition includes the latest techniques in DNA sequencing and genetic engineering of microbial, plant, and animal genomes, including human genome editing, as well as updates across many areas, such as:
* Immunological assays for disease diagnosis, more effective bacteriophage therapy, and new ways of dealing with antibiotic-resistant bacteria
* New and developing vaccines for influenza, tuberculosis, and emerging viral threats, including Zika and SARS-CoV-2
* Engineering bacteria to perform plastic degradation and green algae to produce hydrogen, altering amino acid biosynthesis, and creating designer cellulosomes
* Production of humanized monoclonal antibodies in plants, modifying hybrid plants to produce clonal hybrids, and protecting plants from viral and fungal diseases
Molecular Biotechnology features nearly 600 detailed figures and is an ideal textbook for undergraduate and graduate courses in introductory biotechnology, as well as courses dedicated to utilizing this technology, such as medical, agricultural, environmental, and industrial biotechnology applications.
Inhaltsverzeichnis zu „Molecular Biotechnology “
1.The Development of Molecular Biotechnology 1Emergence of Molecular Biotechnology 1
Recombinant DNA Technology 3
Commercialization of Molecular Biotechnology 6
Concerns and Consequences 8
Summary 10
References 10
Review Questions 11
2.Fundamental Technologies 13
Molecular Cloning 13
Preparation of DNA for Cloning 13
Insertion of Target DNA into a Plasmid Vector 18
Transformation and Selection of Cloned DNA in a Bacterial Host 21
Cloning Eukaryotic Genes 26
Recombinational Cloning 30
Genomic Libraries 32
Genome Engineering Using CRISPR Technology 36
Polymerase Chain Reaction 39
Amplification of DNA by PCR 39
Cloning PCR Products 42
Quantitative PCR 44
Chemical Synthesis of Genes 46
Assembling Oligonucleotides into Genes 46
Assembling PCR Products into Genes 46
DNA Sequencing Technologies 48
Dideoxynucleotide Sequencing 50
Sequencing Using Reversible Chain Terminators 53
Single-Molecule Real-Time Sequencing 55
Nanopore Sequencing 56
Sequencing Whole Genomes 56
Preparation of Genomic DNA Sequencing Libraries 57
High-Throughput Next-Generation Sequencing 59
Genome Sequence Assembly 60
Sequencing Metagenomes 61
Genomics 62
Transcriptomics 65
Proteomics 70
Metabolomics 84
Summary 86
References 87
Review Questions 89
3.Production of Recombinant Proteins 91
Protein Production in Prokaryotic Hosts 91
Regulating Transcription 92
Increasing Translation Efficiency 96
Increasing Protein Stability 100
Increasing Protein Secretion 105
Facilitating Protein Purification 111
Integrating DNA into the Host Chromosome 114
Heterologous Protein
... mehr
Production in Eukaryotic Cells 119
Posttranslational Modification of Eukaryotic Proteins 120
General Features of Eukaryotic Expression Systems 123
Yeast Expression Systems 123
Baculovirus-Insect Cell Expression Systems 137
Mammalian Cell Expression Systems 145
Protein Engineering 156
Directed Mutagenesis 156
Random Mutagenesis 161
Examples of Protein Engineering 164
Summary 173
References 174
Review Questions 176
4.Molecular Diagnostics 179
Immunological Approaches To Detect Protein Biomarkers 180
Antibodies 180
Agglutination 185
Enzyme-Linked Immunosorbent Assays 186
Protein Arrays To Detect Polygenic Diseases 194
Immunoassays for Protein Conformation-Specific Disorders 197
DNA-Based Diagnostic Approaches 199
Hybridization Probes 199
PCR-Based Detection Methods 207
CRISPR-Cas-Based Diagnostic Assays 218
DNA Microarrays 219
Whole-Genome Sequencing To Assess Genetic Disease Risk 225
Detecting RNA Signatures of Disease 226
Detection of Disease-Associated Changes in Gene Expression 227
Detection of RNA Signatures of Antibiotic Resistance in Bacteria 228
Detection of miRNA Signatures of Disease 230
Biofluorescent and Bioluminescent Systems 233
Fluorescent Proteins 233
Luciferase 234
Microbial Biosensors 235
Summary 238
References 239
Review Questions 241
5.Protein Therapeutics 243
Pharmaceuticals 244
Posttranslational Modification of Eukaryotic Proteins 120
General Features of Eukaryotic Expression Systems 123
Yeast Expression Systems 123
Baculovirus-Insect Cell Expression Systems 137
Mammalian Cell Expression Systems 145
Protein Engineering 156
Directed Mutagenesis 156
Random Mutagenesis 161
Examples of Protein Engineering 164
Summary 173
References 174
Review Questions 176
4.Molecular Diagnostics 179
Immunological Approaches To Detect Protein Biomarkers 180
Antibodies 180
Agglutination 185
Enzyme-Linked Immunosorbent Assays 186
Protein Arrays To Detect Polygenic Diseases 194
Immunoassays for Protein Conformation-Specific Disorders 197
DNA-Based Diagnostic Approaches 199
Hybridization Probes 199
PCR-Based Detection Methods 207
CRISPR-Cas-Based Diagnostic Assays 218
DNA Microarrays 219
Whole-Genome Sequencing To Assess Genetic Disease Risk 225
Detecting RNA Signatures of Disease 226
Detection of Disease-Associated Changes in Gene Expression 227
Detection of RNA Signatures of Antibiotic Resistance in Bacteria 228
Detection of miRNA Signatures of Disease 230
Biofluorescent and Bioluminescent Systems 233
Fluorescent Proteins 233
Luciferase 234
Microbial Biosensors 235
Summary 238
References 239
Review Questions 241
5.Protein Therapeutics 243
Pharmaceuticals 244
... weniger
Autoren-Porträt von Bernard R. Glick, Cheryl L. Patten
About the AuthorsBernard R. Glick, PhD, is a distinguished professor emeritus at the University of Waterloo, Ontario, Canada, where he was the chair of biology from 2002 to 2008. He has authored more than 400 publications that have garnered over 54,000 citations.
Cheryl L. Patten, PhD, is a professor of microbiology in the Biology Department at the University of New Brunswick, Fredericton, Canada. She teaches courses in introductory biology, microbiology, and biotechnology.
Bibliographische Angaben
- Autoren: Bernard R. Glick , Cheryl L. Patten
- 2022, 6. Aufl., 896 Seiten, Maße: 22,5 x 28,5 cm, Gebunden, Englisch
- Verlag: Wiley & Sons
- ISBN-10: 1683673646
- ISBN-13: 9781683673644
- Erscheinungsdatum: 15.03.2022
Sprache:
Englisch
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