Avian Influenza Virus

Avian Influenza Virus

Table of Contents

1. A brief introduction to avian influenza virus Erica Spackman

2. Avian Influenza Virus Sample Types, Collection, and Handling Mary-Lea Killian

3. Avian influenza virus RNA extraction from tissue and swab material Erica Spackman and David L. Suarez

4. Type-A Influenza Virus Detection and Quantitation by Real-time RT-PCR Erica Spackman and David L. Suarez

5. Detection and Identification of the H5 Hemagglutinin subtype by Real-time RT-PCR Erica Spackman and David L. Suarez

6. Avian influenza virus isolation and propagation in chicken eggs Peter R. Woolcock

7. Hemagglutination Assay for Avian Influenza Virus Mary-Lea Killian

8. Hemagglutination-Inhibition Test for Avian Influenza Virus Subtype Identification and the Detection and Quantititation of Serum Antibodies to Avian Influenza Virus Janice C. Pedersen

9. Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Subtype or Neuraminidase Antibody Specificity Janice C. Pedersen

10. Immunohistochemical Staining for the Detection of Avian Influenza Virus in Tissues Mary Pantin-Jackwood

11. Wild bird Surveillance for Avian Influenza Virus Justin D. Brown and David E. Stallknecht

12. Reverse Genetics of Avian Influenza Virus Chang-Won Lee and David L. Suarez

13. Evaluating the Cell Mediated Immune Response of Avian Species to Avian Influenza Viruses Darrell R. Kapczynski

14. Measurment of avian cytokines with real-time RT-PCR following infection with avian influenza virus Darrell R. Kapczynski and Michael H. Kogut

From the reviews:"This small book focuses on the laboratory methods used to study the avian influenza strains. ... this book is very timely. ... would be most useful to scientists researching the spread and genetics of avian influenza. ... is a useful book for laboratories needing a quick reference on several difficult laboratory techniques. It is comprehensive enough to answer the difficult questions ... ." (Rebecca T. Horvat, Doody's Review Service, May, 2008)"This book focuses on both the standard virological methods that are fundamental for influenza virus research and diagnostics ... . Each chapter has been supplied with a brief summary, and a rich list of references, tables, graphs and photographs. The book is concluded by a Subject Index. This book may be recommended to all who need extensive information about field and laboratory work with avian influenza viruses, since many important protocols/procedures have not been described in such detail elsewhere." (G. Russ, Folia Microbiologica, Vol. 53, November, 2008)