Basic Cloning Procedures
(Sprache: Englisch)
This manual introduces the reader to basic methods used in the isolation, cloning and analysis of genetic material. The protocols include PT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific...
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This manual introduces the reader to basic methods used in the isolation, cloning and analysis of genetic material. The protocols include PT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein-DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis.Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.
Inhaltsverzeichnis zu „Basic Cloning Procedures “
- Juris Steinbergs, Aleksander Tsimanis: cDNA Synthesis and Cloning.- Eriks Jankevics: DNA Sequencing. - Elita Avota, Normunds Licis: RNA in Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase.
- Janis Klovins, Valdis Berzins: PCR-Based Site-Specific Mutagenesis.
- Indrikis Muiznieks, Nils Rostoks: Analysis of Specific Protein--DNA Interactions.
- Illar Pata, Erkki Truve: Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids.
- Irina Sominskaya, Kaspars Tars: Immunological Methods for Analysis of Recombinant Proteins.
- Tiina Tamm, Erkki Truve: Protein Synthesis in Cell-Free Systems..
Bibliographische Angaben
- 1998, 169 Seiten, 24 Schwarz-Weiß-Abbildungen, Maße: 23,5 cm, Kartoniert (TB), Englisch
- Ed. by Valdis Berzins
- Verlag: Springer Berlin
- ISBN-10: 3540635394
- ISBN-13: 9783540635390
Sprache:
Englisch
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