Basic Concepts on 3D Cell Culture

Chapter 1. Introduction to 3D cell culture.- Chapter 2. Lab equipment for 3D cell culture.- Chapter 3. A view from the cellular perspective.- Chapter 4. Biological, natural and synthetic 3D matrices.- Chapter 5. Hydrogels for 3D cell culture.- Chapter 6. Vascularization in 3D cell culture.- Chapter 7. Application of scaffold-free 3D models.- Chapter 8. 10.Microfluidic Systems and Organ (Human) on a Chip.- Chapter 9. 3D-Bioprinting.- Chapter 10. Non-destructive and label-free monitoring of 3D cell constructs.

Cornelia Kasper studied Chemistry at the University Hannover and received her Diploma degree 1994. After PhD at the Institute for Technical Chemistry 1998 she was employed as EU liason officer at the University of Hannover. In 2007 she received Habilitation. 2000-2011 Cornelia Kasper has been head of Cell Culture and Tissue Engineering Group at the Institute for Technical Chemistry, Leibniz University of Hannover and head of young researcher group JRG "Large Scale Cultivation" within Cluster of Excellence "Rebirth" (from Regenerative Biology and Reconstructive Therapies). Since October 2011 Cornelia Kasper has been appointed as University Professor "Cell and Tissue Culture Technology" at the Department of Biotechnology at BOKU. The focus in her research group is the isolation, characterization, expansion and differentiation of human primary/progenitor and stem cells. For the expansion and guided differentiation she develops optimized strategies and bioprocesses for 3 D cell culture conditions mimicking physiological environment generating functional cells for tissue engineering and cell based therapies and 3 D test systems under defined and controlled dynamic conditions.

Antonina Lavrentieva is a group leader at the Institute of Technical Chemistry, Leibniz University of Hannover, working in the field of stem cell research, biotesting and tissue engineering. In her second PhD Thesis, she studied methods of expanding mesenchymal stem cells (MSCs) in bioreactors, as well as the influence of hypoxia on the MSCs. She studied Biology and Life Science at Moscow State University and the University of Hannover. She also defended a PhD Thesis in Physiology. Her current research interests include stem cell media optimization, 3D cell culture, gradient hydrogels for studying cell niches and 3D-bioprinting.

Dominik Egger is a postdoctoral