Benson's Microbiological Applications Complete Version

Part 1 Microscopy
1 Brightfield Microscopy
2 Darkfield Microscopy
3 Phase-Contrast Microscopy
4 Fluorescence Microscopy
5 Microscopic Measurements
Part 2 Survey of Microorganisms
6 Protozoa, Algae and Cyanobacteria
7 Ubiquity of Bacteria
8 The Fungi: Molds and Yeasts
Part 3 Manipulation of Microorganisms
9 Aseptic Technique
10 Pure Culture Techniques
Part 4 Staining and Observations of Microorganisms
11 Smear Preparation
12 Simple Staining
13 Negative Staining
14 Capsular Staining
15 Gram Staining
16 Spore Staining: Two Methods
17 Acid-Fast Staining: Kinyoun Method
18 Motility Determination
Part 5 Culture Methods
19 Culture Media Preparation
20 Preparation of Stock Cultures
21 Enumeration of Bacteria: The Standard Plate Count
22 Slide Culture: Fungi
Part 6 Bacterial Viruses
23 Determination of a Bacteriophage Titer
24 Burst Size Determination: A One-Step Growth Curve
25 Isolation of Phage from Flies
26 Phage Typing
Part 7 Environmental Influences and Control of Microbial Growth
27 Effects of Oxygen on Growth
28 Temperature: Effects on Growth
29 pH and Microbial Growth
30 Water Activity and Osmotic Pressure
31 Ultraviolet Light: Lethal Effects
32 The Effects of Lysozyme on Bacterial Cells
33 Evaluation of Alcohol: Its Effectiveness as an Antiseptic
34 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method
35 Evaluation of Antiseptics: The Filter Paper Disk Method
36 Effectiveness of Hand Scrubbing
Part 8 Identification of Unknown Bacteria
37 Morphological Study of An Unknown Bacterium
38 Cultural Characteristics
39 Physiological Characteristics: Oxidation and Fermentation Stet Reactions
40 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions
41 Physiological Characteristics: Multiple Test Media
42 Use of Bergey's Manual
Part 9 Miniaturized Multitest Systems
43 Enterobacteriaceae Identification: The API 20E System
44 Enterobacteriaceae

Professor and Chair, Consultant Environmental Microbiology