Bioluminescent Imaging
Methods and Protocols
(Sprache: Englisch)
Bioluminescent Imaging: Methods and Protocols distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology,...
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Bioluminescent Imaging: Methods and Protocols distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology, neurology, infectious diseases, immunology, and others, the detailed chapters of this volume are arranged by topic and describe practical procedures and applications of different bioluminescent reporters, from photoproteins (Aequorin) to bacterial luciferases as well as other secreted (such as Gaussia) and non-secreted luciferases (such as Firefly). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols aims to provide diverse and comprehensive techniques to researchers interested in implementing bioluminescence-based imaging in their laboratory, regardless of their previous level of experience with such methodologies.
Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols aims to provide diverse and comprehensive techniques to researchers interested in implementing bioluminescence-based imaging in their laboratory, regardless of their previous level of experience with such methodologies.
Klappentext zu „Bioluminescent Imaging “
Bioluminescent Imaging: Methods and Protocols distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology, neurology, infectious diseases, immunology, and others, the detailed chapters of this volume are arranged by topic and describe practical procedures and applications of different bioluminescent reporters, from photoproteins (Aequorin) to bacterial luciferases as well as other secreted (such as Gaussia) and non-secreted luciferases (such as Firefly). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols aims to provide diverse and comprehensive techniques to researchers interested in implementing bioluminescence-based imaging in their laboratory, regardless of their previous level of experience with such methodologies.
Inhaltsverzeichnis zu „Bioluminescent Imaging “
1. Bioluminescence Imaging: Basics and Practical Limitations, Christian E. BadrPart I: In Vitro Bioassays
2. Extraction and Quantification of Adenosine Triphosphate in Mammalian Tissues and Cells, Junji Chida and Hiroshi Kido
3. Neuronal Network Imaging in Acute Slices Using Ca2+ Sensitive Bioluminescent Reporter, Ludovic Tricoire and Bertrand Lambolez
4. Gaussia Luciferase-Based Mycoplasma Detection Assay in Mammalian Cell Culture, M. Hannah Degeling,, M. Sarah S. Bovenberg, Marie Tannous, and Bakhos A. Tannous
Part II: Imaging Molecular and Cellular Events
5. Split Gaussia Luciferase for Imaging Ligand-Receptor Binding, Kathryn E. Luker and Gary D. Luker
6. Video-Rate Bioluminescence Imaging of Protein Secretion from a Living Cell, Takahiro Suzuki and Satoshi Inouye
7. Bioluminescence Reporter Gene-Based Detection of MicroRNAs, Hae Young Ko, Young Sik Lee, and Soonhag Kim
8. Monitoring of Transcriptional Dynamics of HIF and NF?B Activities, Miguel A.S. Cavadas and Alex Cheong
9. Real-Time Bioluminescent Tracking of Cellular Population Dynamics, Dan Close, Tingting Xu, Steven Ripp, and Gary Sayler
10. Fabrication of Bioluminescent Capsules and Live-Cell Imaging, Sung Bae Kim
Part III: Imaging of Disease (Pathogenesis)
11. The Bioluminescent Imaging of Spontaneously Occurring Tumors in Immunocompetent
ODD-Luciferase Bearing Transgenic Mice, Scott J. Goldman and Shengkan Jin
12. Blood-Based Assay with Secreted Gaussia Luciferase to Monitor Tumor Metastasis, Hiroshi Yamashita, Dan T. Nguyen, and Euiheon Chung
13. Bioluminescence Imaging of Fungal - Biofilm Development in Live Animals, Greetje Vande Velde, Sona Kucharikova, Patrick Van Dijck, and Uwe Himmelreich
14. Bioluminescent Imaging of Bacteria During Mouse Infection, Jonathan M. Warawa and Matthew B. Lawrenz
Part IV: Therapeutic Imaging
15. Cell-Based Bioluminescence Screening Assays, Romain J. Amante and Christian E. Badr
16. Bioluminescence-Based Monitoring of Virus Vector-Mediated
... mehr
Gene Transfer in Mice, Casey A. Maguire
17. Simultaneous In Vivo Monitoring of Regulatory and Effector T Lymphocytes Using Secreted Gaussia Luciferase, Firefly Luciferase, and Secreted Alkaline Phosphatase, Grant K. Lewandrowski, Ciara N. Magee, Marwan Mounayar, Bakhos A. Tannous, and Jamil Azzi
Part V: Emerging Technologies
18. Multiplex Functional Bioluminescent Reporters Using Gaussia Luciferase Fused to Epitope Tags in an Immunobinding Assay, Sjoerd van Rijn, Thomas Würdinger, and Jonas Nilsson
19. Non-Invasive - In Vivo Monitoring of Extracellular Vesicles, Charles P. Lai, Bakhos A. Tannous, and Xandra O. Breakefield
20. In Vitro and In Vivo Demonstrations of Fluorescence by Unbound Excitation from Luminescence (FUEL), Joe Dragavon, Abdessalem Rekiki, Ioanna Theodorou, Chelsea Samson, Samantha Blazquez, Kelly L. Rogers, Régis Tournebize, and Spencer Shorte
17. Simultaneous In Vivo Monitoring of Regulatory and Effector T Lymphocytes Using Secreted Gaussia Luciferase, Firefly Luciferase, and Secreted Alkaline Phosphatase, Grant K. Lewandrowski, Ciara N. Magee, Marwan Mounayar, Bakhos A. Tannous, and Jamil Azzi
Part V: Emerging Technologies
18. Multiplex Functional Bioluminescent Reporters Using Gaussia Luciferase Fused to Epitope Tags in an Immunobinding Assay, Sjoerd van Rijn, Thomas Würdinger, and Jonas Nilsson
19. Non-Invasive - In Vivo Monitoring of Extracellular Vesicles, Charles P. Lai, Bakhos A. Tannous, and Xandra O. Breakefield
20. In Vitro and In Vivo Demonstrations of Fluorescence by Unbound Excitation from Luminescence (FUEL), Joe Dragavon, Abdessalem Rekiki, Ioanna Theodorou, Chelsea Samson, Samantha Blazquez, Kelly L. Rogers, Régis Tournebize, and Spencer Shorte
... weniger
Bibliographische Angaben
- 2013, 2014, XI, 276 Seiten, 39 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Christian E. Badr
- Verlag: Springer, Berlin
- ISBN-10: 1627037179
- ISBN-13: 9781627037174
Sprache:
Englisch
Rezension zu „Bioluminescent Imaging “
From the reviews:"The intent is to provide diverse group of techniques to everyone interested in implementing bioluminescence-based imaging regardless of their level of experience with such methodologies. These are very worthy objectives and the book successfully meets them. ... The book is illustrated with high-quality pictures and figures. This is an excellent source of protocols and methodologies that use bioluminescence imaging as a tool for visualizing and tracking various biological processes in diverse fields." -- (Omer Iqbal, Doody's Book Reviews, January, 2014)
Pressezitat
From the reviews:"The intent is to provide diverse group of techniques to everyone interested in implementing bioluminescence-based imaging regardless of their level of experience with such methodologies. These are very worthy objectives and the book successfully meets them. ... The book is illustrated with high-quality pictures and figures. This is an excellent source of protocols and methodologies that use bioluminescence imaging as a tool for visualizing and tracking various biological processes in diverse fields." (Omer Iqbal, Doody's Book Reviews, January, 2014)
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