Bioseparations Engineering
Principles, Practice, and Economics
(Sprache: Englisch)
Die Bioseparationstechnik ist eine multidisziplinäre Anwendung technischer und biologischer Prinzipien zum Design von Adsorptionsmitteln, Systemen und Prozessen für die Trennung von Biomolekülen. Dieses praxisorientierte Buch will vor allem Biochemiker und...
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Die Bioseparationstechnik ist eine multidisziplinäre Anwendung technischer und biologischer Prinzipien zum Design von Adsorptionsmitteln, Systemen und Prozessen für die Trennung von Biomolekülen. Dieses praxisorientierte Buch will vor allem Biochemiker und Chemiker anhand zahlreicher Beispiele und Fallstudien in die industrielle Umsetzung der Verfahren einführen. Neben den naturwissenschaftlichen und technischen Grundlagen werden auch ökonomische Aspekte wie die Abschätzung von Kosten einzelner Prozeduren ausführlich besprochen.
Klappentext zu „Bioseparations Engineering “
Multidisciplinary resource for graduate studies and the biotechnology industryKnowledge of the genetic basis of biological functioning continues to grow at an astronomical rate, as do the challenges and opportunities of applying this information to the production of therapeutic compounds, specialty biochemicals, functional food ingredients, environmentally friendly biocatalysts, and new bioproducts from renewable resources. While genetic engineering of living organisms transforms the science of genomics into treatments for cancer, diabetes, and heart disease, or products for industry and agriculture, the science and technology of bioseparations are the keys to delivering these products in a purified form suitable for use by people.
The methods, theory, and materials that reduce the science of bioseparations to practice, whether in the laboratory or the plant, are the subjects of Bioseparations Engineering. Examples address purification of biomolecules ranging from recombinant proteins to gene therapy products, with footnotes detailing economics of the products. Mechanistic analysis and engineering design methods are given for:
* Isocratic and gradient chromatography
* Sedimentation, centrifugation, and filtration
* Membrane systems
* Precipitation and crystallization
Topics addressed within this framework are: stationary phase selection; separations development; modeling of ion exchange, size exclusion, reversed phase, hydrophobic interaction, and affinity chromatography; the impact of regulatory issues on chromatography process design; organization of separation strategies into logical sequences of purification steps; and bridges between molecular biology, combinatorial methods, and separations science.
A result of teaching and developing the subject matter over ten years, Bioseparations Engineering is an ideal text for graduate students, as well as a timely desk book for process engineers, process scientists, researchers, and research associates in the
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pharmaceutical, food, and life sciences industries.
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Inhaltsverzeichnis zu „Bioseparations Engineering “
Preface.Acknowledgements.
Bioseparations.
Sedimentation, Centrifugation, and Filtration.
Membrane Separations.
Precipitation, Crystallization, and Extraction.
Principles of Liquid Chromatography.
Liquid Chromatography Scale-Up.
Principles of Gradient Elution Chromatography.
Principles of Bioseparations for Biopharmaceuticals and Recombinant Protein Products.
Affinity Chromatography: Bridge Between Molecular Biology, Combinatorial Methods, and Separations Science.
Author Index.
Subject Index.
Autoren-Porträt von Michael R. Ladisch
MICHAEL R. LADISCH, PhD, is Distinguished Professor in the Department of Agricultural and Biological Engineering and the Department of Biomedical Engineering, and the Director of the Laboratory of Renewable Resources Engineering, at Purdue University in West Lafayette, Indiana.
Bibliographische Angaben
- Autor: Michael R. Ladisch
- 2001, 1. Auflage, XXIV, 736 Seiten, mit Abbildungen, Maße: 16,1 x 24 cm, Gebunden, Englisch
- Verlag: Wiley & Sons
- ISBN-10: 0471244767
- ISBN-13: 9780471244769
- Erscheinungsdatum: 13.03.2001
Sprache:
Englisch
Rezension zu „Bioseparations Engineering “
"a comprehensive text that has brought together the theory and practice of bioseperations in an intelligent and well-presented fashion." (Bioseperations Engineering (Food & Bioproducts Processing, December 2001)
Pressezitat
"a comprehensive text that has brought together the theory and practice of bioseperations in an intelligent and well-presented fashion." (Bioseperations Engineering (Food & Bioproducts Processing, December 2001)"...Ladish reviews the techniques that have been developed over the past couple decades...in the hope that the explanations will apply to bioproducts not yet invented and biological molecules not yet produced...as well as to those currently being used." (SciTech Book News, Vol. 25, No. 4, December 2001)
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