C. elegans
Methods and Applications
(Sprache: Englisch)
Molecular biology has driven a powerful reductionist, or "molecule-c- tric," approach to biological research in the last half of the 20th century. Red- tionism is the attempt to explain complex phenomena by defining the functional properties of the...
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Klappentext zu „C. elegans “
Molecular biology has driven a powerful reductionist, or "molecule-c- tric," approach to biological research in the last half of the 20th century. Red- tionism is the attempt to explain complex phenomena by defining the functional properties of the individual components of the system. Bloom (1) has referred to the post-genome sequencing era as the end of "naïve reductionism. " Red- tionist methods will continue to be an essential element of all biological research efforts, but "naïve reductionism," the belief that reductionism alone can lead to a complete understanding of living organisms, is not tenable. Organisms are clearly much more than the sum of their parts, and the behavior of complex physiological processes cannot be understood simply by knowing how the parts work in isolation. Systems biology has emerged in the wake of genome sequencing as the s- cessor to reductionism (2-5). The "systems" of systems biology are defined over a wide span of complexity ranging from two macromolecules that interact to carry out a specific task to whole organisms. Systems biology is integrative and seeks to understand and predict the behavior or "emergent" properties of complex, multicomponent biological processes. A systems-level characteri- tion of a biological process addresses the following three main questions: (1) What are the parts of the system (i. e.
Inhaltsverzeichnis zu „C. elegans “
- An Overview of C. elegans Biology; Kevin Strange- Comparative Genomics in C. elegans, C. briggsae and Other Caenorhabditis Species; Avril Coghlan, Jason E. Stajich and Todd W. Harris
- WormBase: Methods for Data Mining and Comparative Genomics; Todd W. Harris and Lincoln D. Stein
- C. elegans Deletion Mutant Screening; Robert J. Barstead and Donald G. Moerman
- Insertional Mutagenesis in Caenorhabditis elegans Using the Drosophila Transposon Mos1: A Method for the Rapid Identification of Mutated Genes; Jean-Louis Bessereau
- SNP Mapping; M. Wayne Davis and Marc Hammarlund
- Creation of Transgenic Lines Using Microparticle Bombardment Methods; Vida Praitis
- Construction of Plasmids for RNAi and In Vitro Transcription of dsRNA; Lisa Timmons
- Delivery Methods for RNAi in Caenorhabditis elegans; Lisa Timmons
- Functional Genomic Approaches in C. elegans; Todd Lamitina
- Assays for Toxicity Studies in C. elegans with Bt Crystal Proteins; Larry J. Bischof, Danielle L. Huffman and Raffi V. Aroian
- Fluorescent Reporter Methods; Harald Hutter
- Electrophysiological Analysis of Neuronal and Muscle Function in C. elegans; Michael M. Francis and Andres Villu Maricq
- Sperm and Oocyte Isolation Methods for Biochemical and Proteomic Analysis; Michael A. Miller
- Preservation of C. elegans Tissue via High-Pressure Freezing and Freeze-Substitution for Ultrastructural Analysis and Immuno-Cytochemistry; Robby M. Weimer
- Intracellular pH Measurements In Vivo Using Green Fluorescent Protein Variants; Keith Nehrke
- Automated Imaging of C. elegans Behavior; Christopher J. Cronin, Zhaoyang Feng and William R. Schafer
- Intracellular Ca2+ Imaging in Caehorhabditis elegans; Rex A. Kerr and William R. Schafer
- In Vitro Culture of C. elegans Somatic Cells
Bibliographische Angaben
- 2006, 292 Seiten, Maße: 23,5 cm, Gebunden, Englisch
- Herausgegeben von Strange, Kevin
- Verlag: Humana Press
- ISBN-10: 1588295974
- ISBN-13: 9781588295972
Sprache:
Englisch
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