Calpain

Single-Step Purification of Calpain-1, Calpain-2, and Calpastatin using Anion Exchange Chromatography.- Bacterial Expression and Purification of Calpains.- Casein Zymography for Analysis of Calpain-1 and Calpain-2 Activity.- FRET-Based Assays to Determine Calpain Activity.- In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transfer.- Determining Temporal and Spatial Expression of Calpains in Amphibians.- Immunohistochemical Localization of Calpains in the Amphibian Xenopus laevis.- Expression and Activity of Calpain A in Drosophila melanogaster.- Use of the beta-Glucoronidase Reporter System (GUS) to Localize Promoter Activities of the Endogenous Plant Calpain DEFECTIVE KERNEL1 (DEK1).- LabCaS for Ranking Potential Calpain Substrate Cleavage Sites from Amino Acid Sequence.- CalCleaveMKL: A Tool for Calpain Cleavage Prediction.- Identification of Calpain-Activated protein Functions.- Production and Purification of Recombinant Calpastatin.- Isolation of Endogenous Calpastatin.- Immunoblotting for Calpastatin Expression.- Experimental Manipulation of Calpain Activity In Vitro.- In Vivo Calpain Knockdown using Delivery of siRNA.- In Vivo Expression of Mutant Calpains in the Eye using Lentivirus.- Methods of Calpain Inhibition to Determine the Role of Calpains in Embryo Development in Amphibians.- Genetic Models of Calpain Deficiency and Ectopic Expression.