Cardiovascular Proteomics
Methods and Protocols
(Sprache: Englisch)
This cutting-edge book presents protocols and strategies for proteomic evaluation of cardiovascular disease written by pioneering researchers in the field. Topics explored in this comprehensive volume include obtaining specific heart proteins, techniques...
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Produktinformationen zu „Cardiovascular Proteomics “
Klappentext zu „Cardiovascular Proteomics “
This cutting-edge book presents protocols and strategies for proteomic evaluation of cardiovascular disease written by pioneering researchers in the field. Topics explored in this comprehensive volume include obtaining specific heart proteins, techniques for identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, and phage display techniques for deciphering the molecular diversity of blood vessels.
Inhaltsverzeichnis zu „Cardiovascular Proteomics “
PartI. Analysis of Heart Proteome- Two-dimensional Polyacrylamide Gel Electrophoresis for Cardiovascular Proteomics
- Michael J. Dunn
- Analyzing The Cardiac Muscle Proteome By Liquid Chromatography-Mass Spectrometry (Lc-ms) Based Expression Proteomics
- Anthony O. Gramolini, Thomas Kislinger, Peter Liu, David H. Maclennan, and Andrew Emili
- 2-D Differential Gel Electrophoresis (DIGE) of Rat Heart Proteins in Ischemia and Ischemia-Reperfusion
- Jun Sakai, Hironori Ishikawa, Hideshi Satoh, Setsuko Yamamoto, Shinichi Kojima, and Masaharu Kanoaka
- Analysis of Antihypertensive Drugs in the Heart of Animal Models: A Proteomic Approach
- Alberto Lázaro, Julio Gállego-delgado, Julio Osende, Jesús Egido, and Fernando Vivanco
- A Solubility Optimization Protocol for 2-D Gel Electrophoresis of Cardiac Tissue
- Brian A. Stanley and Jennifer E. Van Eyk
- A Method for Effective Depletion of Albumin From Cellular Extracts: Application to Human Myocardium
- Brian A. Stanley and Jennifer E. Van Eyk
PartII. Heart Subproteomes
- Subcellular Fractionation
- Mariana E. Guimarães De Araújo and Alfons Huber
- Subfractionation of Heart Tissue: The "In Sequence" Myofilament Protein Extraction Of Myocardial Tissue
- Lesley A. Kane, Irina Neverova, and Jennifer E. Van Eyk
- Optimization of Cardiac Troponin I Pull-Down by Idm Affinity Beads and Seldi-tofT
- Diane E. Bovenkamp, Brian A. Stanley and Jennifer E.van Eyk
- Proteomic Analysis Of The Subunit Composition of Complex I (Nadh:Ubiquinone Oxidoreductase) From Bovine Heart Mitochondria
- Ian M. Fearnley, Joe Carrol and John E. Walker
- Identification of Targets of Phosphorylation in Heart Mitochondria
- Roberta A. Gottliebie
PartIII. Proteomics of Human Atheroma Plaques
- Characterization of The Human Atheroma Plaque Secretome by Proteomic Analysis
- Mari-Carmen Durán, Jose L. Martin-Ventura, Sebastián Mas, Maria G. Barderas, Verónica M. Dardé, Ole N. Jensen, Jesús Egido, and
... mehr
Fernando Vivanco
- Characterization of Hsp-27 Phosphorylation Sites in Human Atherosclerotic Plaque Secretome
- Mari-Carmen Durán, Elisabetta Boeri-Erba, Shabaz Mohammed, Jose L. Martín-Ventura, Jesús Egido, Fernando Vivanco, and Ole N. Jensen
- Western Array Analysis of Human Atherosclerotic Plaque
- Wim Martinetar
PartIV. The Proteome of Cells of The Cardiovascular System
- The Proteome of Endothelial Cells
- Jesús Gonzalez-Cabrero, Mayte Pozo, Mari-Carmen Durán, Rosario de Nicolás, Jesús Egido, and Fernando Vivanco
- Proteomic Study of Caveolae and Rafts Isolated from Human Endothelial Cells
- Richard R. Sprenger and Anton J. G. Horrevoets
- Proteomic Identification of S-nitrosylated Proteins In Endothelial Cells
- Antonio Martínez-Ruiz and Santiago Lamasgo
- The Proteome and Secretome of Human Arterial Smooth Muscle Cell
- Annabelle Dupont and Florence Pinet
- Real-Time in Vivo Proteomic Identification Of Novel Kinase Substrates in Smooth Muscle
- Anne A. Wooldridge and Timothy A. Haystead
- Proteomic Analysis of Vascular Smooth Muscle Cells Treated With Ouabain
- Alexy V. Pshezhetsky
- Isolation and Culture of Adult Mouse Cardiac Myocytes
- Timothy D. OŽConnell, Manoj C. Rodrigo, and Paul C. Simpson
- Proteomic Analysis Of Foam Cells
- Peng-Yuang Yang, Yao-cheng Rui, Peng-Yuan Yang, and Yan-Ling Yuin
- Isolation of The Platelet Releasate
- Judith Coppinger, Desmond J. Fitzgerald, and Patricia B. Maguire
- Enrichment Of Phospho-Tyrosine Proteome Of Human Platelets By Immunoprecipitation
- Martina Foy, Donald F. Harney, Kieran Wynne, and Patricia B. Maguire
- Chapter 25
- Verónica M. Dardé, Mari-Carmen Durán, Jesús Egido, and Fernando Vivanco
PartV. Cardiovascular Biomarkers in Plasma
- Cardiovascular Biomarkers Discovery By Seldi-tof Mass Spectrometry
- Olivier Meilhac, Sandrine Delbosc, and Jean-Baptiste Michel
- Detection of Biomarkers of Stroke Using Seldi-tofe
- Jean-charles Sanchez, Pierre Lescuyer, Denis Hochstrasser, and Laure Allard
- Depletion of High-Abundance Proteins In Plasma by Immunoaffinity Substraction For Two-Dimensional Difference Gel Electrophoresis (DIGE) Analysis
- Verónica M. Dardé, Maria G. Barderas, and Fernando Vivanco
- A Rapid, Economic and Reproducible Method For Human Serum Delipidation, Albumin And IgG Removal, For Proteomic Analysis
- Qin Fu, Diane E. Bovenkamp And Jennifer E.van Eyk
PartVI. Bioinformatics: Emerging Technologies
- Mitoproteome: Human Heart Mitochondrial Protein Sequence Database
- Purnima Guda, Shankar Subramaniam, and Chittibabu Guda
- Techniques To Decipher Molecular Diversity By Phage Display
- Dawn R. Christianson, Michael G. Ozawa, Renata Pasqualini, and Wadih Arapraprap
- Characterization of Hsp-27 Phosphorylation Sites in Human Atherosclerotic Plaque Secretome
- Mari-Carmen Durán, Elisabetta Boeri-Erba, Shabaz Mohammed, Jose L. Martín-Ventura, Jesús Egido, Fernando Vivanco, and Ole N. Jensen
- Western Array Analysis of Human Atherosclerotic Plaque
- Wim Martinetar
PartIV. The Proteome of Cells of The Cardiovascular System
- The Proteome of Endothelial Cells
- Jesús Gonzalez-Cabrero, Mayte Pozo, Mari-Carmen Durán, Rosario de Nicolás, Jesús Egido, and Fernando Vivanco
- Proteomic Study of Caveolae and Rafts Isolated from Human Endothelial Cells
- Richard R. Sprenger and Anton J. G. Horrevoets
- Proteomic Identification of S-nitrosylated Proteins In Endothelial Cells
- Antonio Martínez-Ruiz and Santiago Lamasgo
- The Proteome and Secretome of Human Arterial Smooth Muscle Cell
- Annabelle Dupont and Florence Pinet
- Real-Time in Vivo Proteomic Identification Of Novel Kinase Substrates in Smooth Muscle
- Anne A. Wooldridge and Timothy A. Haystead
- Proteomic Analysis of Vascular Smooth Muscle Cells Treated With Ouabain
- Alexy V. Pshezhetsky
- Isolation and Culture of Adult Mouse Cardiac Myocytes
- Timothy D. OŽConnell, Manoj C. Rodrigo, and Paul C. Simpson
- Proteomic Analysis Of Foam Cells
- Peng-Yuang Yang, Yao-cheng Rui, Peng-Yuan Yang, and Yan-Ling Yuin
- Isolation of The Platelet Releasate
- Judith Coppinger, Desmond J. Fitzgerald, and Patricia B. Maguire
- Enrichment Of Phospho-Tyrosine Proteome Of Human Platelets By Immunoprecipitation
- Martina Foy, Donald F. Harney, Kieran Wynne, and Patricia B. Maguire
- Chapter 25
- Verónica M. Dardé, Mari-Carmen Durán, Jesús Egido, and Fernando Vivanco
PartV. Cardiovascular Biomarkers in Plasma
- Cardiovascular Biomarkers Discovery By Seldi-tof Mass Spectrometry
- Olivier Meilhac, Sandrine Delbosc, and Jean-Baptiste Michel
- Detection of Biomarkers of Stroke Using Seldi-tofe
- Jean-charles Sanchez, Pierre Lescuyer, Denis Hochstrasser, and Laure Allard
- Depletion of High-Abundance Proteins In Plasma by Immunoaffinity Substraction For Two-Dimensional Difference Gel Electrophoresis (DIGE) Analysis
- Verónica M. Dardé, Maria G. Barderas, and Fernando Vivanco
- A Rapid, Economic and Reproducible Method For Human Serum Delipidation, Albumin And IgG Removal, For Proteomic Analysis
- Qin Fu, Diane E. Bovenkamp And Jennifer E.van Eyk
PartVI. Bioinformatics: Emerging Technologies
- Mitoproteome: Human Heart Mitochondrial Protein Sequence Database
- Purnima Guda, Shankar Subramaniam, and Chittibabu Guda
- Techniques To Decipher Molecular Diversity By Phage Display
- Dawn R. Christianson, Michael G. Ozawa, Renata Pasqualini, and Wadih Arapraprap
... weniger
Bibliographische Angaben
- 2007, 440 Seiten, Maße: 16 x 24,1 cm, Gebunden, Englisch
- Herausgegeben: Fernando Vivanco
- Verlag: Humana Press
- ISBN-10: 1588295354
- ISBN-13: 9781588295354
- Erscheinungsdatum: 05.02.2007
Sprache:
Englisch
Rezension zu „Cardiovascular Proteomics “
"...a fine collection of methods and protocols currently employed in cardiovascular proteomics that will be of great use to all who are invovled in cardiovascular research." -Proteomics Clin. Appl."...a thorough set of protocols for the cardiac biology community. Most laboratories will have some of these in place, but can profit from the broad coverage of this book." -Doody's Book Review, Weighted Numerical Score: 76 - 3 stars
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