Cell-Based Assays Using iPSCs for Drug Development and Testing
(Sprache: Englisch)
The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues. Chapters in this book cover topics such as requirements and preconditions for...
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Klappentext zu „Cell-Based Assays Using iPSCs for Drug Development and Testing “
The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues. Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Based Assay using iPSCs for Drug Development and Testing: Methods and Protocols is a valuable resource for researchers working in this important and developing field of study.Inhaltsverzeichnis zu „Cell-Based Assays Using iPSCs for Drug Development and Testing “
Preface...Table of Contents...Contributing Authors...1. Cell-Based Assays using Differentiated Human Induced Pluripotent CellsM Z. Cader, M. Graf, M. Burcin, C.F. Mandenius, and J.A. RossPart I Differentiation of iPC to Target Cells for Assays 2. Monolayer Generation of Vascular Endothelial Cells from Human Pluripotent Stem CellsKlaus Christensen, Filip Roudnicky, Mark Burcin, and Christoph Patsch3. Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease ModellingKatharina Janssen, Lamiaa Bahnassawy, Claudia Kiefer, Jürgen Korffmann, Georg C. Terstappen, Viktor Lakics, Miroslav Cik, and Peter Reinhardt4. Human Induced Pluripotent Stem Cell-Derived Definitive Endoderm Bulk Culture and Hepatic DifferentiationAnwar A. Palakkan, Jyoti Nanda, and James A. Ross5. Production of Cardiomyocytes from Human Pluripotent Stem Cells by Bioreactor TechnologiesCaroline Halloin, Michelle Coffee, Felix Manstein, and Robert Zweigerdt6. Improved Protocol of Cardiac Differentiation and Maturation of Pluripotent Stem CellsImen Jebeniani, Shunli Ding, and Michel Pucéat7. Human Pluripotent Stem Cell Expansion in Stirred Tank BioreactorsFelix Manstein, Caroline Halloin, and Robert Zweigerdt8. A Protocol for the One-Step Differentiation of Human Induced Pluripotent Stem Cells into Mature PodocytesCormac Murphy, Elisabeth Feifel, Paul Jennings, Gerhard Gstraunthaler, and Anja Wilmes9. Differentiation of hiPS Cells into Definitive Endoderm for High Throughput ScreeningTeresa Bluhmki, Stefanie Traub, Eva Schruf, James Garnett, Florian Gantner, Daniel Bischoff, and Ralf HeilkerPart II High Throughput Metabolomics and Proteomic Cell-Based Assays10. LC-MS/MS Based Metabolomics for Cell CulturesAnna Artati, Cornelia Prehn, and Jerzy Adamski11. Preparation of iPSCs for Targeted Proteomic AnalysisAnna Baud, Wendy E. Heywood, Daniel Little, Paul Gissen, and Kevin Mills12. Measurement of Bile Acids as a Marker of the Functionality of iPSC-Derived
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HepatocytesFrancesca Mazzacuva, Maëlle Lorvellec, Agostino Cilibrizzi, Kevin Mills, Peter Clayton, and paul Gissen13. Mass Spectrometry Measurement of Albumin/Alpha Fetoprotein Ratio as an Indicator of iPSC-Derived Hepatocyte DifferentiationFrancesca Mazzacuva, Maëlle Lorvellec, Agostino Cilibrizzi, Kevin Mills, Wendy Heywood, Peter Clayton, and Paul GissenPart III High Content Imaging14. Seeding Induced Pluripotent Stem Cell-Derived Neurons onto 384-Well PlatesDaniel Little, Christin Luft, Oliver Pezzini-Picart, Olukunbi Mosaku, Robin Ketteler, Michael J. Devine, and Paul Gissen15. High Content Autophagy Analysis in iPSC-Derived Neurons using Immuno-FluorescenceDaniel Little, Christin Luft, Olukunbi Mosaku, Robin Ketteler, Michael J. Devine, and Paul Gissen16. High Content Analysis of Mitochondrial Function in iPSC-Derived NeuronsDaniel Little, Christin Luft, Olukunbi Mosaku, Robin Ketteler, Michael J. Devine, and Paul Gissen17. Evaluating the Effect of Drug Compounds on Cardiac Spheroids using the Cardiac Cell Outgrowth AssayJonas Christoppersson, Florian Meier, Henning Kempf, Kristin Schwanke, Michelle Coffee, Mario Beilmann, Robet Zweigerdt, and Carl-Fredrik MandeniusPart IV Electrophysiology Assays18. Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes using Multi-Well Microelectrode ArraysUdo Kraushaar and Elke Guenther19. Multielectrode Array (MEA) Based Detection of Spontaneous Network Activity in Human iPSC-Derived Cortical NeuronsValeria Kizner, Sandra Fischer, and Maximilian NaujockPart V Miscellaneous Cell-Based Assays20. Assessing Neuronal Excitability on a Fluorometric Imaging Plate Reader (FLIPR) Following a Defined Electrostimulation ParadigmValeria Kizner, Sandra Fischer, Benjamin Jähnke, and Maximilian Naujock21. Fabrication of a Microfluidic Cell Culture Device using Photolithographic and Soft Lithographic TechniquesJonas Christoffersson and Carl-Fredrik Mandenius22. Using a Microfluidic Device for Culture and Drug Toxicity Testing of 3D CellsJonas Christoffersson and Carl-Fredrik Mandenius23. hiPS Cell-Derived Neurons for High-Throughput ScreeningStefanie Traub and Ralf Heilker
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Bibliographische Angaben
- 2019, 1st ed., 266 Seiten, mit farbigen Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben von Mandenius, Carl-Frederik; Ross, James A.
- Herausgegeben: James A. Ross, Carl-Fredrik Mandenius
- Verlag: Springer, New York
- ISBN-10: 149399476X
- ISBN-13: 9781493994762
- Erscheinungsdatum: 25.05.2019
Sprache:
Englisch
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