Cell Migration in Inflammation and Immunity

Chemotaxis and Interaction with Vascular or Lymphatic Endothelium.- Analysis of Integrin-Dependent Rapid Adhesion Under Laminar-Flow Conditions.- Posttranslational Processing of Chemokines.- Chemotactic Profiling of Lymphocyte Subpopulations.- Measurement of the Levels of Polymerized Actin (F-Actin) in Chemokine-Stimulated Lymphocytes and GFP-Coupled cDNA Transfected Lymphoid Cells by Flow Cytometry.- Evaluation of Rho Family Small G-Protein Activity Induced by Integrin Ligation on Human Leukocytes.- Reconstructing Leukocyte Migration in 3D Extracellular Matrix by Time-Lapse Videomicroscopy and Computer-Assisted Tracking.- Analyzing Chemotaxis Using Dictyostelium discoideum as a Model System.- Conditional Transgenic Models to Study Chemokine Biology.- Intravital Microscopy as a Tool for Studying Recruitment and Chemotaxis.- Tracking Antigen-Specific Lymphocytes In Vivo.- Analysis of Homing-Receptor Expression on Infiltrating Leukocytes in Disease States.- Interaction of Viral Chemokine Inhibitors with Chemokines.- Discovery of Small-Molecule Antagonists of Chemokine Receptors.- Visualization and Analysis of Adhesive Events in Brain Microvessels by Using Intravital Microscopy.- Animal Models to Study Chemokine Receptor Function In Vivo.- Assessing the Role of Multiple Phosphoinositide 3-Kinases in Chemokine Signaling.- In Vitro and In Vivo Models to Study Chemokine Regulation of Angiogenesis.- Real-Time In Vitro Assay for Studying Chemoattractant-Triggered Leukocyte Transendothelial Migration Under Physiological Flow Conditions.- Generation of Monoclonal Antibodies Against Chemokine Receptors.- Detection of High-Affinity ?4-Integrin Upon Leukocyte Stimulation by Chemoattractants or Chemokines.