Culture of Animal Cells
A Manual of Basic Technique
(Sprache: Englisch)
The most complete resource on the techniques, equipment,principles, and practices of animal cell cultureSince publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue...
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The most complete resource on the techniques, equipment,principles, and practices of animal cell culture
Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced.
The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes:
* An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols
* New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering
* An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment
* Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text
* Fully updated references
* The clearest, most consistent presentation of step-by-step protocols available
* Numerous diagrams, photographs, tables, and charts
* Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites
Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.
Klappentext zu „Culture of Animal Cells “
Ein äußerst populäres, vielfach bewährtes Buch - die "Bibel" der Kultur tierischer Zellen! Diese neue Auflage wurde um die aktuellsten Informationen aus den Gebieten der Biologie und Biotechnologie, der Medien und Spezialtechniken, des DNA-Transfers und der Tumorkultur ergänzt. Neue Kapitel beschäftigen sich unter anderem mit Biofermentatoren und der Immortalisierung von Kulturen. Auf zehn Farbseiten wird ein Atlas tierischer Zellen geboten.
Inhaltsverzeichnis zu „Culture of Animal Cells “
List of Figures.List of Color Plates.Preface.Abbreviations.1. Introduction.
2. Training Programs.
3. Biology of Cultured Cells.
4. Laboratory Design and Layout.
5. Equipment.
6. Aseptic Technique.
7. Safety, Bioethics, and Validation.
8. Culture Vessels and Substrates.
9. Defined Media and Supplements.
10. Serum-Free Media.
11. Preparation and Sterilization.
12. Primary Culture.
13. Subculture and Cell Lines.
14. Cloning and Selection.
15. Cell Separation.
16. Characterization.
17. Differentiation.
18. Transformation and Immortalization.
19. Contamination.
20. Cryopreservation.
21. Quantitation.
22. Cytotoxocity.
23. Culture of Specific Cell Types.
24. Culture of Tumor Cells.
25. Organotypic Culture.
26. Scale-Up.
27. Specialized Techniques.
28. Problem Solving.
29. In Conclusion.
Appendix I: Preparation of Reagents.
Appendix II: Sources of Equipment and Materials.
AppendixIII: Suppliers and Other Resources.
Appendix IV: Glossary.
Appendix V: General Textbooks and Relevant Journals.
References.Index.
Autoren-Porträt von Robert I. Freshney
R. IAN FRESHNEY, PhD, is an honorary senior research fellow in the Centre for Oncology and Applied Pharmacology, part of the Cancer Research UK Beatson Laboratories at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique.
Bibliographische Angaben
- Autor: Robert I. Freshney
- 2005, 5th ed., XXVI, 642 Seiten, mit farbigen Abbildungen, Maße: 19 x 23,7 cm, Gebunden, Englisch
- Verlag: Wiley & Sons
- ISBN-10: 0471453293
- ISBN-13: 9780471453291
Sprache:
Englisch
Rezension zu „Culture of Animal Cells “
"...Freshney provides 28 chapters that reflect the latest progress in media, specialized techniques, biotechnology, DNA transfer and tumor culture." (SciTech Book News, March 2001)"Labs that intensively make use of cell culture, will find Culture of Animal Cells a helpful reference and great training source." --Yale Journal of Biology and Medicine, 74, 2001.
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