Cytoskeleton Methods and Protocols

Long-Term Live Cell Imaging of Cell Migration: Effects of Pathogenic Fungi on Human Epithelial Cell Migration.- Live-Cell Imaging of Mitochondria and the Actin Cytoskeleton in Budding Yeast.- Imaging of the Actin Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells.- Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells.- Imaging Cytoskeleton Components by Electron Microscopy.- Purification and Localization of Intraflagellar Transport Particles and Polypeptides.- Fluorescence Imaging of the Cytoskeleton in Plant Roots.- Microtubules in Plant Cells: Strategies and Methods for Immunofluorescence, Transmission Electron Microscopy, and Live Cell Imaging.- Basic Methods to Visualize Actin Filaments In Vitro Using Fluorescence Microscopy for Observation of Filament Severing and Bundling.- An In Vitro Model System to Test Mechano-Microbiological Interactions Between Bacteria and Host Cells.- Reconstitution of a Minimal Actin Cortex by Coupling Actin Filaments to Reconstituted Membranes.- Use of Nanobodies to Localize Endogenous Cytoskeletal Proteins and to Determine Their Contribution to Cancer Cell Invasion by Using an ECM Degradation Assay.- Actin-Dynamics in Plant Cells: The Function of Actin Perturbing Substances: Jasplakinolide, Chondramides, Phalloidin, Cytochalasins, and Latrunculins.- Quantitative Motion Analysis in Two and Three Dimensions.- Measurement of Cell Motility Using Microgrooved Substrates.- The Study of Cell Motility by Cell Traction Force Microscopy (CTFM).- Melanosome Motility in Fish Retinal Pigment Epithelial (RPE) Cells.- Analysis of Stem Cell Motility In Vivo Based on Immunodetection of Planarian Neoblasts and Tracing of BrdU-Labeled Cells After Partial Irradiation.- Chemotaxis: Under Agarose Assay.- Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila.- Proteomic Analysis of Cytoskeleton Proteins in Fish.- Using a Hand-Held Gene Gun forGenetic Transformation of Tetrahymena