Development of Low Cost Media for Fungal and Bacterial Plant Pathogens

This book deals with study of Development of Low Cost Media for Fungal and Bacterial Plant Pathogens. To isolate, culture and maintenance of microorganism, cultural mediums are required. These cultural medium are prepared in research laboratories and also now-a-days readily available in the market. However their enormous cost restricts their use by the scientist of developing countries. The mediums and their constituents are costly and may not be available at remote place laboratories. Therefore the research workers are always in search of cheap and alternative sources of the medium. The replacement of quantity of agar and sucrose, the carbohydrates and protein sources of the medium are therefore a challenging task. Further development of universal medium for fungal and bacterial plant pathogen was necessarily in need. To replace quantity of agar, gelling agents like tapioca and psyllium husk was used. 1% agar with 2% tapioca was a good solidified gelling agent for bacterial streaking as well as placement of fungal disc. The combination of these solidified gelling agent favoured a good bacterial growth as well as fungal growth.