DNA Damage Detection In Situ, Ex Vivo, and In Vivo

In Situ Detection of Apoptosis by the TUNEL Assay: An Overview of Techniques.-Combination of TUNEL Assay with Immunohistochemistry for Simultaneous Detection of DNA Fragmentation and Oxidative Cell Damage.- EM-ISEL: A Useful Tool to Visualize DNA Damage at the Ultrastructural Level.- In Situ Labeling of DNA Breaks and Apoptosis by T7 DNA Polymerase.- In Situ Ligation: a Decade and a Half of Experience.-In Situ Ligation Simplified: Using PCR Fragments for Detection of Double-Strand DNA Breaks in Tissue Sections.- 5'OH DNA Breaks in Apoptosis and their Labeling by Topoisomerase-Based Approach.- Detection of DNA Strand Breaks in Apoptotic Cells by Flow- and Image-Cytometry.- Fluorochrome-Labeled Inhibitors of Caspases (FLICA): Convenient In Vitro and In Vivo Markers of Apoptotic Cells for Cytometric Analysis.- Combining Fluorescent In Situ Hybridization with the Comet Assay for Targeted Examination of DNA Damage and Repair. Simultaneous Labeling of Single- and Double-Strand DNA Breaks by DNA Breakage Detection-FISH (DBD-FISH).- Co-Localization of DNA Repair Proteins with UV-induced DNA Damage in Locally Irradiated Cells.- Ultrasound Imaging of Apoptosis: Spectroscopic Detection of DNA-Damage Effects at High and Low-Frequencies. - Quantifying Etheno-DNA Adducts in Human Tissues, White Blood Cells and Urine by Ultrasensitive 32P-postlabeling and Immunohistochemistry.- ELISpot Assay as a Tool to Study Oxidative Stress in Peripheral Blood Mononuclear Cells.- Cytokinesis-Block Micronucleus Cytome assay in Lymphocytes.- Buccal Micronucleus Cytome Assay.- -H2AX Detection in Peripheral Blood Lymphocytes, Splenocytes, Bone Marrow, Xenografts and Skin.- Immunologic Detection of Benzo(a)pyrene-DNA Adducts.- Non-Invasive Assessment of Oxidatively Damaged DNA: Liquid Chromatography-Tandem Mass Spectrometry Analysis of Urinary 8-Oxo-7,8-dihydro-2'-deoxyguanosine.- Assessing sperm DNA fragmentation with the Sperm Chromatin Dispersion (SCD) test.

From the reviews:"This volume is presenting the major essays used nowadays in cell and molecular biology laboratories to detect in situ, ex vivo and in vivo DNA damages. Enjoyable and interesting enough is the long explanation devoted by Prof. Didenko ... to the use of the terms in vivo, ex vivo and in situ ... . Well done! ... . Those of us interested in whatsoever way to DNA damage studies will surely find this book very useful." (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)

From the reviews:"This volume is presenting the major essays used nowadays in cell and molecular biology laboratories to detect in situ, ex vivo and in vivo DNA damages. Enjoyable and interesting enough is the long explanation devoted by Prof. Didenko ... to the use of the terms in vivo, ex vivo and in situ ... . Well done! ... . Those of us interested in whatsoever way to DNA damage studies will surely find this book very useful." (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)