Dünnschicht-Chromatographie

INTRODUCTION
- What is TLC?
- When is TLC used?
- Where is TLC used
- How is the result of a TLC represented?
- What kinds of reference substances are used in TLC?
- The literature on TLC

PRECOATED LAYERS
- Why use precoated layers?
- How are precoated layers produced?
- What kinds of precoated layers are there?
- What are precoated layers used for?
- Criteria for choosing stationary phases in TLC
- Effect of the stationary phase when the solvents are identical
- Recommendations for the procurement and storage of precoated layers
- Problems in the nomenclature and classification of precoated layers

BEFORE TLC DEVELOPMENT
- Handling of the precoated layers
- Prewashing
- Activation
- Conditioning
- Impregnation
- Application of the sample
- Positioning of the sample
- Drying before development

SOLVENT, DEVELOPING CHAMBERS AND DEVELOPMENT
- Solvent
- TLC developing chambers
- Development of thin-layer chromatograms
- Drying after development

EVALUATION WITHOUT DERIVATIZATION
- Visual direct evaluation
- Optical direct evaluation using special equipment
- Coupling methods for substance identification
- Documentation without or before derivatization

DERIVATIZATION
- Thermochemical reaction
- Irradiation with high energy light
- Reaction with reagents
- Special types of derivatization
- Treatment of derivatized chromatograms

EVALUATION AFTER DERIVATIZATION
- Visual evaluation
- Evaluation by TLC scanner
- Evaluation by video system
- Evaluation by flat-bed scanner

DOCUMENTATION
- Description of a thin-layer chromatogram
- Documentation by reproduction, tracing or photocopying
- Photographic documentation
- Video documentation
- TLC scanner documentation
- Flat-bed scanner documentation

GMP/GLP-CONFORMING TLC WORK
- Validation of TLC methods
- Use of qualified
- calibrated equipment
- GMP/GLP-conforming rawdata sheets
- Examples of GMP/GLP-conforming testing procedures

LOADING EFFECTS
- Definite