Electroporation Protocols

Dec 1st, 2006 Editor: Shulin Li (USA) Book title: Electroporation protocols: Experimental and Clinical Gene Medicine Publisher: The Humana Press, Inc Preface (Shulin Li, no need to fit chapter format) I. Basic concepts of DNA transfer via electroporation (review chapters, no need to fit chapter format for all the chapters in this section) Application of Electroporation gene therapy: past, current, and future (Lluis M. Mir) Mechanism by which electroporation mediates DNA migration and entry into cells and the targeted tissues (Marie-Pierre Rols) Applicator and electrode design for in vivo DNA delivery by electroporation (Dietmar Rabussay) Electrode assemblies used for electroporation of cultured cells (Leda Raptis and Kevin L. Firth) Formulations for DNA delivery via electroporation in vivo (Khursheed Anwer) Overview of drug delivery and alternative methods to electroporation (Sek-Wen Hui) Nano-Electroporation-A First Look (Raji Sundararajan) II. In vitro targeted gene delivery via electroporation Nucleofection of Human Embryonic Stem Cells (Henrike Siemen, Lars Nolden, Stefanie Terstegge, Philipp Koch, and Oliver Brustle) Delivery of whole tumor lysate into dendritic cells for cancer vaccination (Linda N. Liu, Rama Shivakumar, Cornell Allan, and Joseph C. Fratantoni) Delivery of tumor antigen encoding mRNA into dendritic cells for vaccination (Annelies Michiels, Sandra Tuyaerts, Aude Bonehill, Carlo Heirman, Jurgen Corthals, and Kris Thielemans) Delivery of DNA into natural killer cells for immunotherapy (Kathrin Schoenberg, Hans-Ingo Trompeter, and Markus Uhrberg) Electroporation ofadherent cells in situ for the study of signal transduction and gap junctional communication (Leda Raptis, Adina Vultur, Heather L. Brownell, Evangelia Tomai, Aikaterini Anagnostopoulou, and Kevin L. Firth) Delivery of DNA into adipocytes within adipose tissue (James G. Granneman) III: In vivo targeted gene delivery via electroporation Delivery of DNA into muscle for