Epitope Mapping Protocols

Preface...Table of Contents...Contributing Authors... 1. An Introduction to Epitope MappingJohan Nilvebrant and Johan Rockberg Part I Whole Antigen Methods 2. Epitope Mapping of Antibody-Antigen Interactions with X-Ray CrystallographyMoeko Toride and Cory L. Brooks 3. Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical ConsiderationsLuca Simonelli, Mattia Pedotti, Marco Bardelli, Simon Jurt, Oliver Zerbe, and Luca Varani 4. Label-Free Cell-Based Assay for Characterization of Biomolecules and ReceptorsDiluka Peiris and Lars M. Jørgensen 5. Probing Antibody Binding Sites on G Protein-Coupled Receptors Using Genetically-Encoded Photo-Activatable Cross-LinkersThomas Huber and Thomas P. Sakmar 6. Rapid Mapping of Protein Binding Sites and Conformational Epitopes by Coupling Yeast Surface Display to Chemical Labeling and Deep SequencingTariq Ahmad Najar, Shruti Khare, and Raghavan Varadarajan 7. Epitope Mapping Using Yeast Display and Next Generation SequencingThomas Van Blarcom, Andrea Rossi, Davide Foletti, Purnima Sundar, Steven Pitts, Zea Melton, Dilduz Telman, Lora Zhao, Wai Ling Cheung, Jan Berka, Wenwu Zhai, Pavel Strop, Jaume Pons, Arvind Rajpal, and Javier Chaparro-Riggers Part II Protein Domain and Fragment-Based Methods 8. B-Cell Epitope Mapping Using a Library of Overlapping Synthetic Peptides in an Enzyme-Linked Immunosorbent AssayThiru Vanniasinkam, Mary D. Barton, Tongted Phumoonna Das, and Michael W. Heuzenroeder 9. Array-In-Well Epitope Mapping of Phage-Displayed AntibodiesUrpo Lamminmäki, Gaurav Batra, and Petri Saviranta 10. Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment LibrariesAnna-Luisa Volk, Francis Jingxin Hu, and Johan Rockberg 11. Discontinuous Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Folded DomainsAnna-Luisa Volk and Johan Rockberg Part III Real-Time Based Methods 12. Kinetic Analysis and Epitope Binning Using Surface Plasmon ResonanceJohan Nilvebrant 13. Epitope